{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:37:20Z","timestamp":1782409040781,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Malignant pleural mesothelioma (MPM) is associated with asbestos exposure. Asbestos can induce chronic inflammation which in turn can lead to silencing of tumour suppressor genes. Wnt signaling pathway can be affected by chronic inflammation and is aberrantly activated in many cancers including colon and MPM.<jats:italic>SFRP<\/jats:italic>genes are antagonists of Wnt pathway, and<jats:italic>SFRP<\/jats:italic>s are potential tumour suppressors in colon, gastric, breast, ovarian, and lung cancers and mesothelioma. This study investigated the expression and DNA methylation of<jats:italic>SFRP<\/jats:italic>genes in MPM cells lines with and without demethylation treatment. Sixty-six patient FFPE samples were analysed and have showed methylation of<jats:italic>SFRP2<\/jats:italic>(56%) and<jats:italic>SFRP5<\/jats:italic>(70%) in MPM.<jats:italic>SFRP2<\/jats:italic>and<jats:italic>SFRP5<\/jats:italic>tumour-suppressive activity in eleven MPM lines was confirmed, and long-term asbestos exposure led to reduced expression of the<jats:italic>SFRP1<\/jats:italic>and<jats:italic>SFRP<\/jats:italic>2 genes in the mesothelium (MeT-5A) via epigenetic alterations. Finally, DNA methylation of<jats:italic>SFRP<\/jats:italic>s is detectable in MPM patient plasma samples, with methylated<jats:italic>SFRP2<\/jats:italic>and<jats:italic>SFRP5<\/jats:italic>showing a tendency towards greater abundance in patients. These data suggested that<jats:italic>SFRP<\/jats:italic>genes have tumour-suppresive activity in MPM and that methylated DNA from<jats:italic>SFRP<\/jats:italic>gene promoters has the potential to serve as a biomarker for MPM patient plasma.<\/jats:p>","DOI":"10.1155\/2017\/2536187","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T23:30:40Z","timestamp":1513207840000},"page":"1-10","source":"Crossref","is-referenced-by-count":17,"title":["<i>SFRP<\/i>Tumour Suppressor Genes Are Potential Plasma-Based Epigenetic Biomarkers for Malignant Pleural Mesothelioma"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7060-0624","authenticated-orcid":true,"given":"Yuen Yee","family":"Cheng","sequence":"first","affiliation":[{"name":"Asbestos Diseases Research Institute (ADRI), University of Sydney, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Mok","sequence":"additional","affiliation":[{"name":"Asbestos Diseases Research Institute (ADRI), University of Sydney, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Tan","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Leygo","sequence":"additional","affiliation":[{"name":"Asbestos Diseases Research Institute (ADRI), University of Sydney, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"McLaughlin","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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