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Although expression of miR-200s is aberrant in liver fibrosis, its role in liver fibrogenesis still remains unknown. Here, we investigated the role of miR-200c in the activation of human hepatic stellate cells (HSCs) and induction of liver fibrosis.<jats:italic> Methods<\/jats:italic>. We engineered human HSCs (LX2 cell line) to stably express miR-200c (LX2-200c) or empty vector control (LX2-nc).<jats:italic> Results<\/jats:italic>. miR-200c expression upregulated <jats:italic>\u03b1<\/jats:italic>-smooth muscle actin (SMA) and vimentin, enhanced HSCs growth and migration, increased expression of collagen type I (a main component of ECM) gene and secretion of epidermal growth factor (EGF), and upregulated the phosphorylation of Akt, a downstream effector of the PI3K pathway. As a target of miR-200s and inhibitor of PI3K pathway, FOG2 protein expression was significantly suppressed in LX2-200c cells. Moreover, LY294002, a highly selective inhibitor of PI3K, blocked phosphorylation of Akt and the effects of miR-200c.<jats:italic> Conclusions<\/jats:italic>. These data suggest that miR-200c activates HSCs in liver fibrosis possibly by downregulating FOG2 protein expression and upregulating PI3K\/Akt signaling. Autocrine activation of EGF signaling may also be a mechanism of miR-200c-mediated HSCs activation. So miR-200c can be a potential marker for HSCs activation and liver fibrosis progression, as well as a potential target to attenuate liver fibrosis.<\/jats:p>","DOI":"10.1155\/2017\/2670658","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T17:02:41Z","timestamp":1497373361000},"page":"1-8","source":"Crossref","is-referenced-by-count":21,"title":["miR-200c Accelerates Hepatic Stellate Cell-Induced Liver Fibrosis via Targeting the FOG2\/PI3K Pathway"],"prefix":"10.1155","volume":"2017","author":[{"given":"Tengfei","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Liver Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China"},{"name":"Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, China"},{"name":"Institute of Cancer Stem Cell, Dalian Medical University, Dalian, 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