{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:14:35Z","timestamp":1762431275738,"version":"3.37.3"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["MST105-2221-E-182A-003","CMRPG3D0071-2","G3A019"],"award-info":[{"award-number":["MST105-2221-E-182A-003","CMRPG3D0071-2","G3A019"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"publisher","award":["MST105-2221-E-182A-003","CMRPG3D0071-2","G3A019"],"award-info":[{"award-number":["MST105-2221-E-182A-003","CMRPG3D0071-2","G3A019"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"publisher","award":["MST105-2221-E-182A-003","CMRPG3D0071-2","G3A019"],"award-info":[{"award-number":["MST105-2221-E-182A-003","CMRPG3D0071-2","G3A019"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>This study aimed to identify predictors of success rate of mesenchymal stem cell (MSC) isolation from human deciduous teeth pulp. A total of 161 deciduous teeth were extracted at the dental clinic of Chang Gung Memorial Hospital. The MSCs were isolated from dental pulps using a standard protocol. In total, 128 colonies of MSCs were obtained and the success rate was 79.5%. Compared to teeth not yielding MSCs successfully, those successfully yielding MSCs were found to have less severe dental caries (no\/mild-to-moderate\/severe: 63.3\/24.2\/12.5% versus 12.5\/42.4\/42.4%,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>) and less frequent pulpitis (no\/yes: 95.3\/4.7% versus 51.5\/48.5%,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>). In a multivariate regression model, it was confirmed that the absence of dental caries (OR = 4.741, 95% CI = 1.564\u201314.371,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.006<\/mml:mn><\/mml:math>) and pulpitis (OR = 9.111, 95% CI = 2.921\u201328.420,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.001<\/mml:mn><\/mml:math>) was significant determinants of the successful procurement of MSCs. MSCs derived from pulps with pulpitis expressed longer colony doubling time than pulps without pulpitis. Furthermore, there were higher expressions of proinflammatory cytokines, interleukin- (IL-) 6 and monocyte chemoattractant protein- (MCP-) 1,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>, and innate immune response [toll-like receptor 1 (TLR1) and TLR8,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.05<\/mml:mn><\/mml:math>; TLR2, TLR3, and TLR6,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>] in the inflamed than noninflamed pulps. Therefore, a carious deciduous tooth or tooth with pulpitis was relatively unsuitable for MSC processing and isolation.<\/jats:p>","DOI":"10.1155\/2017\/2851906","type":"journal-article","created":{"date-parts":[[2017,3,9]],"date-time":"2017-03-09T16:04:37Z","timestamp":1489075477000},"page":"1-9","source":"Crossref","is-referenced-by-count":20,"title":["Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp"],"prefix":"10.1155","volume":"2017","author":[{"given":"Aileen I.","family":"Tsai","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiang-Hsi","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6078-1396","authenticated-orcid":true,"given":"Wey-Ran","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jen-Fen","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih-Chun","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Far Eastern Memorial Hospital, New Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I-Kuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, China Medical University Hospital and College of Medicine, China Medical University, Taichung, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Hung","family":"Huang","sequence":"additional","affiliation":[{"name":"Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Hao","family":"Weng","sequence":"additional","affiliation":[{"name":"Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Wei","family":"Hsu","sequence":"additional","affiliation":[{"name":"Poison Center, Department of Nephrology, Chang Gung Memorial Hospital and College of Medicine, Chang Gung University, Linkou, 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