{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T12:22:38Z","timestamp":1772540558421,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Pterygium is a relatively common eye disease that can display an aggressive clinical behaviour. To evaluate the<jats:italic> in vitro<\/jats:italic> effects of<jats:italic> Curcuma longa<\/jats:italic> on human pterygium-derived keratinocytes, specimens of pterygium from 20 patients undergoing pterygium surgical excision were collected. Pterygium explants were put into culture and derived keratinocytes were treated with an alcoholic extract of 1.3%<jats:italic> Curcuma longa<\/jats:italic> in 0.001% Benzalkonium Chloride for 3, 6, and 24\u2009h. Cultured cells were examined for CAM5.2 (anti-cytokeratin antibody) and CD140 (anti-fibroblast transmembrane glycoprotein antibody) expression between 3th and 16th passage to assess cell homogeneity. TUNEL technique and Annexin-V\/PI staining in flow cytometry were used to detect keratinocyte apoptosis. We showed that<jats:italic> Curcuma longa <\/jats:italic>exerts a proapoptotic effect on pterygium-derived keratinocytes already after 3\u2009h treatment. Moreover, after 24\u2009h treatment,<jats:italic> Curcuma longa <\/jats:italic>induces a significant increase in TUNEL as well as Annexin-V\/PI positive cells in comparison to untreated samples. Our study confirms previous observations highlighting the expression, in pterygium keratinocytes, of nuclear VEGF and gives evidence for the first time to the expression of nuclear and cytoplasmic VEGF-R1. All in all, these findings suggest that<jats:italic> Curcuma longa<\/jats:italic> could have some therapeutic potential in the treatment and prevention of human pterygium.<\/jats:p>","DOI":"10.1155\/2017\/2956597","type":"journal-article","created":{"date-parts":[[2017,12,17]],"date-time":"2017-12-17T18:31:03Z","timestamp":1513535463000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["<i>Curcuma longa<\/i> Is Able to Induce Apoptotic Cell Death of Pterygium-Derived Human Keratinocytes"],"prefix":"10.1155","volume":"2017","author":[{"given":"Silvia","family":"Sancilio","sequence":"first","affiliation":[{"name":"Department of Pharmacy, G. d\u2019Annunzio University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio","family":"Di Staso","sequence":"additional","affiliation":[{"name":"Ophthalmic Clinic, Department of Life, Health and Environmental Sciences, L\u2019Aquila University, Piazzale S. 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