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MicroRNA-16-5p (miR-16-5p) has been reported to be downregulated in lesions of patients with GCT, but little is known about its role in GCT. To explore the underlying function of miR-16-5p in GCT, we first detected its expression in patients with GCT. The results showed that osteoclast formation increased, whereas miR-16-5p expression considerably decreased with the severity of bone destruction. Furthermore, we found that miR-16-5p expression considerably decreased with the progression of receptor activator of nuclear factor-<jats:italic>\u03ba<\/jats:italic>B ligand- (RANKL-) induced osteoclastogenesis. Functionally, miR-16-5p mimics significantly reduced RANKL-induced osteoclast formation. However, treatment with an inhibitor of miR-16-5p significantly promoted osteoclastogenesis. These findings reveal that miR-16-5p inhibits osteoclastogenesis and that it may represent a therapeutic target for giant cell tumor of bone.<\/jats:p>","DOI":"10.1155\/2017\/3173547","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T17:01:30Z","timestamp":1494867690000},"page":"1-6","source":"Crossref","is-referenced-by-count":29,"title":["MicroRNA-16-5p Inhibits Osteoclastogenesis in Giant Cell Tumor of Bone"],"prefix":"10.1155","volume":"2017","author":[{"given":"Shang","family":"Sang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Sixth People\u2019s Hospital, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhichang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Sixth People\u2019s Hospital, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Sixth People\u2019s Hospital, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9324-4685","authenticated-orcid":true,"given":"Changwei","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Orthopaedics and Traumatology, Shanghai Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3773-9346","authenticated-orcid":true,"given":"Yang","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Sixth People\u2019s Hospital, Shanghai Jiao Tong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3094-y"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-014-0289-1"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2013-0432"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-15-0669"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/s1470-2045(13)70277-8"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.12.075"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.09.076"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1007\/BF01606524"},{"issue":"2","key":"9","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0002-9440(10)63323-8","volume":"165","year":"2004","journal-title":"American Journal of Pathology"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.7150\/thno.10305"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17020156"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1111\/os.12231"},{"issue":"10","key":"15","volume":"7","year":"2016","journal-title":"Cell Death & Disease"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4223"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-012-2707-6"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-015-3777-5"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70010-3"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/boneres.2016.14"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1097\/01.bor.0000127825.05580.eb"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/3173547.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/3173547.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/3173547.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T17:01:35Z","timestamp":1494867695000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/3173547\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":21,"alternative-id":["3173547","3173547"],"URL":"https:\/\/doi.org\/10.1155\/2017\/3173547","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}