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Recently we showed that<jats:italic>f17a<\/jats:italic>was found to be the most prevalent and crucial virulent factor among the pathogenic<jats:italic>E. coli<\/jats:italic>isolated from bovine mastitis. Here, in this report, the recombinant F17A based subunit vaccine adjuvant with MF59 was tested for immunogenicity against<jats:italic>E. coli<\/jats:italic>in a murine model. The vaccinated mice did not show any abnormal behavioral changes and histopathological lesions after vaccination. The specific antibody level against F17A was significantly higher in MF59-adjuvant-group, and also lasted for longer duration with a significant<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mo stretchy=\"false\">(<\/mml:mo><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><mml:mo stretchy=\"false\">)<\/mml:mo><\/mml:math>production level of IgG1 and IgG2a. Moreover, we noted higher survival rate in mice injected with F17A-MF59-adjuvant group after challenging with the clinical<jats:italic>E. coli<\/jats:italic>strain<jats:italic>.<\/jats:italic>Our findings of bacterial clearance test revealed that elimination rate from liver, spleen, and kidney in MF59-adjuvant-group was significantly higher than the control group. Finally, the proportion of CD4+T cells was increased, while CD8+ was decreased in MF59-adjuvant group. In conclusion, the current study reveals the capability of F17A-MF59 as a potential vaccine candidate against pathogenic<jats:italic>E. coli<\/jats:italic>causing mastitis in dairy animals.<\/jats:p>","DOI":"10.1155\/2017\/3248483","type":"journal-article","created":{"date-parts":[[2017,12,3]],"date-time":"2017-12-03T07:23:27Z","timestamp":1512285807000},"page":"1-10","source":"Crossref","is-referenced-by-count":13,"title":["Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against<i>Escherichia coli<\/i>Isolated from Bovine Mastitis"],"prefix":"10.1155","volume":"2017","author":[{"given":"Wei","family":"Chen","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shandong Agricultural University, Tai\u2019an 271018, 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