{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:31:44Z","timestamp":1787315504127,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>\n                    Intestinal epithelium represents a protective physical barrier and actively contributes to the mucosal immune system. Polarized basolateral intestinal secretion of inflammatory mediators, followed by activation of NF-\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B signaling and inflammatory pathways in endothelial cells, efficiently triggers extravasation of neutrophils from the vasculature, therefore contributing to the development and maintenance of intestinal inflammation. Proper regulation of NF-\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B activation at the epithelial interface is crucial for the maintenance of physiological tissue homeostasis. Many papers reported that anthocyanins, a group of compounds belonging to flavonoids, possess anti-inflammatory effects and modulate NF-\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B activity. In this study, by using a coculture in vitro system, we aimed to evaluate the effects of TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -stimulated intestinal cells on endothelial cells activation, as well as the protective effects of cyanidin-3-glucoside (C3G). In this model, TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    induced nuclear translocation of NF-\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B and TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    and IL-8 gene expression in Caco-2 cells, whereas C3G pretreatment dose-dependently reduced these effects. Furthermore, TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    -stimulated Caco-2 cells induced endothelial cells activation with increased E-selectin and VCAM-1 mRNA, leukocyte adhesion, and NF-\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B levels in HUVECs, which were inhibited by C3G. We demonstrated that selective inhibition of the NF-\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B pathway in epithelial cells represents the main mechanism by which C3G exerts these protective effects. Thus, anthocyanins could contribute to the management of chronic gut inflammatory diseases.\n                  <\/jats:p>","DOI":"10.1155\/2017\/3454023","type":"journal-article","created":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T16:03:35Z","timestamp":1488989015000},"page":"1-8","source":"Crossref","is-referenced-by-count":72,"title":["Cyanidin-3-O-Glucoside Modulates the In Vitro Inflammatory Crosstalk between Intestinal Epithelial and Endothelial Cells"],"prefix":"10.1155","volume":"2017","author":[{"given":"Daniela","family":"Ferrari","sequence":"first","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8954-4797","authenticated-orcid":true,"given":"Francesco","family":"Cimino","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Fratantonio","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Sofia","family":"Molonia","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romina","family":"Bashllari","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossana","family":"Bus\u00e0","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Saija","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6135-3892","authenticated-orcid":true,"given":"Antonio","family":"Speciale","sequence":"additional","affiliation":[{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s11605-010-1321-9"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400832101"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.4.477"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1021\/jf060300l"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.200500211"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3390\/molecules18067253"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.2174\/092986711797535263"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00922.2012"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01541.x"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2016.10.014"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2015.09.020"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073001"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1021\/jf900567t"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.186345"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/s12263-012-0324-4"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2014.01.048"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1312"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2011.09.017"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1097\/01.MIB.0000225338.14356.d5"},{"key":"20","volume":"31, article e154","year":"2003","journal-title":"Nucleic Acids Research"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1007\/s12263-014-0404-8"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2007.10.001"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1021\/jf1029515"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1021\/jf1008605"},{"key":"26","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.1365-2036.1997.tb00816.x","volume":"11","year":"1997","journal-title":"Alimentary Pharmacology and Therapeutics"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i41.5848"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/925219"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i5.1165"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i5.578"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1021\/jf502180j"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1039\/c2fo30233k"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-55-18"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.540633"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0804647"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201300102"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/3454023.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/3454023.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/3454023.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T04:41:52Z","timestamp":1750048912000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2017\/3454023\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":36,"alternative-id":["3454023","3454023"],"URL":"https:\/\/doi.org\/10.1155\/2017\/3454023","relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}