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However, studies relating to antifibrotic activity have not been reported. Currently, there is no standard treatment for hepatic fibrosis. This study aimed to investigate the antifibrotic activity of TL in human hepatic stellate LX\u20102 cells. Results from cell viability and cell death assays showed that the extract at high concentrations was toxic to LX\u20102 cells. TL extract reversed the transformation of LX\u20102 cells to myofibroblast\u2010like characteristics in response to stimulation by transforming growth factor\u2010beta 1. This action may be associated with the effect of TL in suppressing <jats:italic>\u03b1<\/jats:italic>\u2010SMA and collagen\u2010I production observed by immunofluorescence study and western blot analysis. Additionally, TL extract significantly decreased MMP\u20109 activity which is consistent with the reduction of <jats:italic>MMP-9<\/jats:italic>, <jats:italic>MMP-2<\/jats:italic>, and <jats:italic>TIMP-1<\/jats:italic> gene expression. The effect of TL in suppressing fibrosis may be associated with its ability to inhibit the activation of p38 MAPK and Erk1\/2 kinases as examined by western blot analysis. Our study provides convincing evidence that TL possesses antifibrotic activity which may be through the suppression of TGF\u2010\u03b2<jats:sub>1<\/jats:sub>\u2010mediated production of MMPs, collagen\u20101, and <jats:italic>\u03b1<\/jats:italic>\u2010SMA in hepatic stellate cells.<\/jats:p>","DOI":"10.1155\/2017\/3508569","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T23:31:04Z","timestamp":1513207864000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>Thunbergia laurifolia<\/i> Exhibits Antifibrotic Effects in Human Hepatic Stellate Cells"],"prefix":"10.1155","volume":"2017","author":[{"given":"Ratchadaporn","family":"Namsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noppamas","family":"Rojanasthien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9620-4590","authenticated-orcid":false,"given":"Seewaboon","family":"Sireeratawong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piyanuch","family":"Rojsanga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wutigri","family":"Nimlamool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8951-8532","authenticated-orcid":false,"given":"Saranyapin","family":"Potikanond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,13]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-17558"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-17557"},{"key":"e_1_2_8_3_2","first-page":"225","article-title":"Activated hepatic stellate cells in liver cirrhosis. 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