{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:25:51Z","timestamp":1790814351061,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T00:00:00Z","timestamp":1503964800000},"content-version":"vor","delay-in-days":240,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Schistosomiasis is a disease endemic in several states of Brazil. The population control of the transmitter mollusks is done with Bayluscide WP 70\u00ae, in the control programs. OMS preconize molluscicidal assays using Becker with 500\u2009mL of final volume, restringing the number of natural products and synthetic drugs to be tested in function of high quantity of material necessary. A new technique to assess the toxic effects for <jats:italic>Biomphalaria<\/jats:italic> sp. is the purpose of this work, for developing adaptation for this aquatic organism, using a low volume of test solution in 24\u2010well plates. We used <jats:italic>Biomphalaria glabrata<\/jats:italic> (10\u201312\u2009mm size) in a static system, consisting of the following components: Becker containing 10 snails or 24\u2010well plates where snails were individualized for a volume of 2\u2009mL per well for 24 and 48 hours. For the assays, we added aqueous solutions of Bayluscide WP 70, at a concentration of 1\u20135\u2009mg\/L, distilled water, and 1% dimethyl sulfoxide. Data were evaluated using Kappa\u2019s coefficient, <jats:italic>Z<\/jats:italic> factor validation, and comparison study. This technique to assess the toxic effect has proven to be a useful tool to detect lethal and sublethal effects, which could be used as a new evaluation protocol.<\/jats:p>","DOI":"10.1155\/2017\/3673197","type":"journal-article","created":{"date-parts":[[2017,8,29]],"date-time":"2017-08-29T21:02:03Z","timestamp":1504040523000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A New Technique Using Low Volumes: A New Technique to Assess the Molluscicidal Activity Using Low Volumes"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0714-4608","authenticated-orcid":false,"given":"Jos\u00e9 Augusto Albuquerque","family":"Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane Paix\u00e3o","family":"Cavalcante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo da Silva","family":"Rangel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Claudio Vit\u00f3ria Atico","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8218-834X","authenticated-orcid":false,"given":"Robson Xavier","family":"Faria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,29]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-311X2007000200017"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000900003"},{"key":"e_1_2_9_3_2","volume-title":"Pesticides and Their Application for the Control of Vectors and Pests of Public Health Importance","author":"World Health Organization","year":"2006"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-2208-8_2"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761992000100004"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761992000400014"},{"key":"e_1_2_9_7_2","first-page":"567","article-title":"Molluscicide screening and evaluation","volume":"33","author":"World Health Organization","year":"1965","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_9_8_2","article-title":"Assessment of molluscicidal, cercaricidal and miracicidal activities of crude extracts of ocimum americanum, bridelia micrantha and chenopodium ambrosoides","volume":"5","author":"Victor M. 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