{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:14:23Z","timestamp":1778775263512,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"APHL and CDC","award":["U60HM000803"],"award-info":[{"award-number":["U60HM000803"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tuberculosis Research and Treatment"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>The primary platform used for pyrazinamide (PZA) susceptibility testing of<jats:italic> Mycobacterium tuberculosis<\/jats:italic> is the MGIT culture system (Becton Dickinson). Since false-resistant results have been associated with the use of this system, we conducted a multicenter evaluation to determine the effect of using a reduced cell density inoculum on the rate of false resistance. Two reduced inoculum densities were compared with that prescribed by the manufacturer (designated as \u201cBD\u201d method). The reduced inoculum methods (designated as \u201cA\u201d and \u201cC\u201d) were identical to the manufacturer\u2019s protocol in all aspects with the exception of the cell density of the inoculum. Twenty genetically and phenotypically characterized<jats:italic> M. tuberculosis<\/jats:italic> isolates were tested in duplicate by ten independent laboratories using the three inoculum methods. False-resistant results declined from 21.1% using the standard \u201cBD\u201d method to 5.7% using the intermediate (\u201cA\u201d) inoculum and further declined to 2.8% using the most dilute (\u201cC\u201d) inoculum method. The percentages of the resistant results that were false-resistant declined from 55.2% for the \u201cBD\u201d test to 28.8% and 16.0% for the \u201cA\u201d and \u201cC\u201d tests, respectively. These results represent compelling evidence that the occurrence of false-resistant MGIT PZA susceptibility test results can be mitigated through the use of reduced inoculum densities.<\/jats:p>","DOI":"10.1155\/2017\/3748163","type":"journal-article","created":{"date-parts":[[2017,11,8]],"date-time":"2017-11-08T18:31:07Z","timestamp":1510165867000},"page":"1-9","source":"Crossref","is-referenced-by-count":9,"title":["Using Reduced Inoculum Densities of<i> Mycobacterium tuberculosis<\/i> in MGIT Pyrazinamide Susceptibility Testing to Prevent False-Resistant Results and Improve Accuracy: A Multicenter Evaluation"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0892-1528","authenticated-orcid":true,"given":"Glenn P.","family":"Morlock","sequence":"first","affiliation":[{"name":"Division of Tuberculosis Elimination, Laboratory Branch, National Center for HIV\/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8366-6515","authenticated-orcid":true,"given":"Frances C.","family":"Tyrrell","sequence":"additional","affiliation":[{"name":"Division of Tuberculosis Elimination, Laboratory Branch, National Center for HIV\/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8519-5948","authenticated-orcid":true,"given":"Dorothy","family":"Baynham","sequence":"additional","affiliation":[{"name":"Tennessee Department of Health, Division of Laboratory Services, 630 Hart Lane, Nashville, TN 37216, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4435-6709","authenticated-orcid":true,"given":"Vincent 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