{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T13:15:58Z","timestamp":1776777358097,"version":"3.51.2"},"reference-count":67,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government Program of the Russian Federation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p><jats:italic>Chlamydia trachomatis<\/jats:italic>imposes serious health problems and causes infertility. Because of asymptomatic onset, it often escapes antibiotic treatment. Therefore, vaccines offer a better option for the prevention of unwanted inflammatory sequelae. The existence of serologically distinct serovars of<jats:italic>C. trachomatis<\/jats:italic>suggests that a vaccine will need to provide protection against multiple serovars.<jats:italic>Chlamydia<\/jats:italic>spp. use a highly conserved type III secretion system (T3SS) composed of structural and effector proteins which is an essential virulence factor. In this study, we expressed the T3SS needle protein of<jats:italic>Chlamydia muridarum,<\/jats:italic>TC_0037, an ortholog of<jats:italic>C. trachomatis<\/jats:italic>CdsF, in a replication-defective adenoviral vector (AdTC_0037) and evaluated its protective efficacy in an intravaginal<jats:italic>Chlamydia muridarum<\/jats:italic>model. For better immune responses, we employed a heterologous prime-boost immunization protocol in which mice were intranasally primed with AdTC_0037 and subcutaneously boosted with recombinant TC_0037 and Toll-like receptor 4 agonist monophosphoryl lipid A mixed in a squalene nanoscale emulsion. We found that immunization with TC_0037 antigen induced specific humoral and T cell responses, decreased<jats:italic>Chlamydia<\/jats:italic>loads in the genital tract, and abrogated pathology of upper genital organs. Together, our results suggest that TC_0037, a highly conserved chlamydial T3SS protein, is a good candidate for inclusion in a<jats:italic>Chlamydia<\/jats:italic>vaccine.<\/jats:p>","DOI":"10.1155\/2017\/3865802","type":"journal-article","created":{"date-parts":[[2017,3,26]],"date-time":"2017-03-26T21:01:12Z","timestamp":1490562072000},"page":"1-14","source":"Crossref","is-referenced-by-count":18,"title":["Chlamydial Type III Secretion System Needle Protein Induces Protective Immunity against<i>Chlamydia muridarum<\/i>Intravaginal Infection"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9702-2940","authenticated-orcid":true,"given":"Ekaterina A.","family":"Koroleva","sequence":"first","affiliation":[{"name":"Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health of Russian Federation, Gamaleya Street 18, Moscow 123098, 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