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This study aimed to evaluate tocotrienol\u2010rich fraction (TRF) modulation in reestablishing the oxidative status of myoblasts during replicative senescence and to compare the effects of TRF with other antioxidants (<jats:italic>\u03b1<\/jats:italic>\u2010tocopherol (ATF) and <jats:italic>N<\/jats:italic>\u2010acetyl\u2010cysteine (NAC)). Primary human myoblasts were cultured to young, presenescent, and senescent phases. The cells were treated with antioxidants for 24\u2009h, followed by the assessment of free radical generation, lipid peroxidation, antioxidant enzyme mRNA expression and activities, and the ratio of reduced to oxidized glutathione. Our data showed that replicative senescence increased reactive oxygen species (ROS) generation and lipid peroxidation in myoblasts. Treatment with TRF significantly diminished ROS production and decreased lipid peroxidation in senescent myoblasts. Moreover, the gene expression of superoxide dismutase <jats:italic>(SOD2)<\/jats:italic>, catalase <jats:italic>(CAT),<\/jats:italic> and glutathione peroxidase <jats:italic>(GPX1)<\/jats:italic> was modulated by TRF treatment, with increased activity of superoxide dismutase and catalase and reduced glutathione peroxidase in senescent myoblasts. In comparison to ATF and NAC, TRF was more efficient in heightening the antioxidant capacity and reducing free radical insults. These results suggested that TRF is able to ameliorate antioxidant defense mechanisms and improves replicative senescence\u2010associated oxidative stress in myoblasts.<\/jats:p>","DOI":"10.1155\/2017\/3868305","type":"journal-article","created":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T21:05:16Z","timestamp":1485291916000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Tocotrienol\u2010Rich Fraction Ameliorates Antioxidant Defense Mechanisms and Improves Replicative Senescence\u2010Associated Oxidative Stress in Human Myoblasts"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2493-1761","authenticated-orcid":false,"given":"Shy Cian","family":"Khor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan Zurinah","family":"Wan Ngah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2577-6289","authenticated-orcid":false,"given":"Yasmin Anum","family":"Mohd Yusof","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norwahidah","family":"Abdul Karim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5239-6196","authenticated-orcid":false,"given":"Suzana","family":"Makpol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,24]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2003"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.2.493"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00043.2011"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201x.2005.01417.x"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afq034"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.07.008"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075201"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.11.006"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/830257"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/a:1013122805060"},{"key":"e_1_2_8_11_2","first-page":"363","article-title":"Relationship between antioxidant intakes and class I sarcopenia in elderly men and women","volume":"11","author":"Chaput J. 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