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This open-label, single-arm feasibility study evaluated the safety and therapeutic effectiveness of autologous bone marrow cell (aBMC) concentrate in revascularization of CLI patients utilizing a rapid point-of-care device. Seventeen (17) no-option CLI patients with ischemic rest pain were enrolled in the study. Single dose of aBMC, prepared utilizing an intraoperative point-of-care device, the Res-Q\u2122 60 BMC system, was injected intramuscularly into the afflicted limb and patients were followed up at regular intervals for 12 months. A statistically significant improvement in Ankle Brachial Index (ABI), Transcutaneous Oxygen Pressure (TcPO<jats:sub>2<\/jats:sub>), mean rest pain and intermittent claudication pain scores, wound\/ ulcer healing, and 6-minute walking distance was observed following aBMC treatment. Major amputation-free survival (mAFS) rate and amputation-free rates (AFR) at 12 months were 70.6% and 82.3%, respectively. In conclusion, aBMC injections were well tolerated with improved tissue perfusion, confirming the safety, feasibility, and preliminary effectiveness of aBMC treatment in CLI patients.<\/jats:p>","DOI":"10.1155\/2017\/4137626","type":"journal-article","created":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T10:58:55Z","timestamp":1485946735000},"page":"1-16","source":"Crossref","is-referenced-by-count":13,"title":["Safety and Effectiveness of Bone Marrow Cell Concentrate in the Treatment of Chronic Critical Limb Ischemia Utilizing a Rapid Point-of-Care System"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0869-5591","authenticated-orcid":true,"given":"Venkatesh","family":"Ponemone","sequence":"first","affiliation":[{"name":"TotipotentRX Centre for Cellular Medicine, Subsidiary of Cesca Therapeutics Inc., Gurgaon, 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