{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:25:22Z","timestamp":1782948322129,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007856","name":"Baylor College of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005300","name":"American Chemical Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005300","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Trichuriasis is a disease of poverty for which excretory and secretory (ES) products that induce the protective immunity are being investigated as candidate vaccines antigens. In this study, ES products of<jats:italic>T. muris<\/jats:italic>and immune sera were produced. The immune sera recognized more than 20 proteins on a 2D-gel of ES products of<jats:italic>T. muris<\/jats:italic>adult worms. Tm16 was one of the proteins identified by mass spectrometry. Tm16 shares 57% sequence identity with Ov16, an immunodominant diagnostic antigen from<jats:italic>Onchocerca volvulus<\/jats:italic>. Recombinant Tm16 with a carboxyl terminal hexahistidine was produced using<jats:italic>Pichia pastoris.<\/jats:italic>Polyclonal antibodies against rTm16 were generated by one-prime and two-boost immunization of three female Balb\/c mice with 25\u2009<jats:italic>\u03bc<\/jats:italic>g of recombinant Tm16 emulsified with ISA720 adjuvant. These polyclonal antibodies confirmed that Tm16 is localized to the ES products and the soluble fraction of the adult worm. Additionally, the high-resolution crystal structure of Tm16 was solved by molecular replacement. Tm16 belongs to the phosphatidylethanolamine-binding-like protein (PEBP1) family and this is the first structure of a PEBP1 from a parasite.<\/jats:p>","DOI":"10.1155\/2017\/4342789","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T21:02:14Z","timestamp":1502744534000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["Identification, Characterization, and Structure of Tm16 from<i>Trichuris muris<\/i>"],"prefix":"10.1155","volume":"2017","author":[{"given":"Zhuyun","family":"Liu","sequence":"first","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Kelleher","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanii","family":"Tabb","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfei","family":"Wei","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen","family":"Pollet","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Hotez","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"},{"name":"Departments of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Bottazzi","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"},{"name":"Departments of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6884-9452","authenticated-orcid":true,"given":"Bin","family":"Zhan","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-2700","authenticated-orcid":true,"given":"Oluwatoyin A.","family":"Asojo","sequence":"additional","affiliation":[{"name":"National School of Tropical Medicine, Baylor College of Medicine and Texas Children\u2019s Hospital Center for Vaccine Development, Houston TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI34261"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.02.005"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.02.006"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1001\/jama.299.16.1937"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410130533"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2008.01035.x"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.11.008"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(90)90016-F"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/43686"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.21.7207-7217.2001"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106991200"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002524200"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3619"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307087200"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.4.1770"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.3.589"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0096454"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-1046-4"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01136.x"},{"issue":"1","key":"21","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s00436-002-0815-0","volume":"90","year":"2003","journal-title":"Parasitology Research"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00174.x"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000064957"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000068293"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(92)90449-M"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0641-8"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1107\/S090744499900935X"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905039107"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905001617"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444903028956"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(98)00125-7"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909052925"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg556"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/15.4.305"}],"container-title":["Journal of Parasitology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2017\/4342789.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2017\/4342789.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jpr\/2017\/4342789.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T06:26:38Z","timestamp":1602743198000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jpr\/2017\/4342789\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":34,"alternative-id":["4342789","4342789"],"URL":"https:\/\/doi.org\/10.1155\/2017\/4342789","relation":{},"ISSN":["2090-0023","2090-0031"],"issn-type":[{"value":"2090-0023","type":"print"},{"value":"2090-0031","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}