{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:17:45Z","timestamp":1790234265586,"version":"4.1.0"},"reference-count":84,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"name":"John Hunter Hospital Charitable Trust"},{"name":"School of Medicine and Public Health Research Training Scheme"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p><jats:italic>Background.<\/jats:italic> Regulation of myometrial progesterone receptor (PR) expression is an unresolved issue central to understanding the mechanism of functional progesterone withdrawal and initiation of labor in women.<jats:italic> Objectives.<\/jats:italic> To determine whether pregnant human myometrium undergoes culture-induced changes in<jats:italic> PR<\/jats:italic> isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise<jats:italic> PR<\/jats:italic> isoform expression in culture.<jats:italic> Methods.<\/jats:italic> Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF<jats:sub>2<jats:italic>\u03b1<\/jats:italic><\/jats:sub>, NF-<jats:italic>\u03ba<\/jats:italic>B inhibitors, or TSA. Following 48\u2009h culture,<jats:italic> PR-T<\/jats:italic>,<jats:italic> PR-A<\/jats:italic>, and<jats:italic> PR-B<\/jats:italic> mRNA levels were determined using qRT-PCR.<jats:italic> PR-A<\/jats:italic>\/<jats:italic>PR-B<\/jats:italic> ratios were calculated.<jats:italic> Results. PR-T<\/jats:italic> and<jats:italic> PR-A<\/jats:italic> expression and the<jats:italic> PR-A<\/jats:italic>\/<jats:italic>PR-B<\/jats:italic> ratio significantly increased in culture. Steroids prevented the culture-induced increase in<jats:italic> PR-T<\/jats:italic> and<jats:italic> PR-A<\/jats:italic> expression. Stretch blocked the effects of steroids on<jats:italic> PR-T<\/jats:italic> and<jats:italic> PR-A<\/jats:italic> expression. PMA further increased the<jats:italic> PR-A<\/jats:italic>\/<jats:italic>PR-B<\/jats:italic> ratio, while TSA blocked culture-induced increases of<jats:italic> PR-A<\/jats:italic> expression and the<jats:italic> PR-A<\/jats:italic>\/<jats:italic>PR-B<\/jats:italic> ratio.<jats:italic> Conclusion.<\/jats:italic> Human myometrial tissue in culture undergoes changes in<jats:italic> PR<\/jats:italic> gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and\/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.<\/jats:p>","DOI":"10.1155\/2017\/4589214","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T17:00:38Z","timestamp":1493744438000},"page":"1-17","source":"Crossref","is-referenced-by-count":18,"title":["Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8647-9213","authenticated-orcid":true,"given":"Marina","family":"Ilicic","sequence":"first","affiliation":[{"name":"School of Medicine and Public Health, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW 2308, Australia"},{"name":"Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia"},{"name":"Hunter Medical Research Institute, 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