{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:22:58Z","timestamp":1760955778893,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1A6A3A11933471","R0005797","10051518"],"award-info":[{"award-number":["2016R1A6A3A11933471","R0005797","10051518"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003052","name":"Ministry of Trade, Industry and Energy","doi-asserted-by":"publisher","award":["2016R1A6A3A11933471","R0005797","10051518"],"award-info":[{"award-number":["2016R1A6A3A11933471","R0005797","10051518"]}],"id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003052","name":"Ministry of Trade, Industry and Energy","doi-asserted-by":"publisher","award":["2016R1A6A3A11933471","R0005797","10051518"],"award-info":[{"award-number":["2016R1A6A3A11933471","R0005797","10051518"]}],"id":[{"id":"10.13039\/501100003052","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"Objectives<\/jats:italic>. As a homing factor of stem cell, stromal derived factor-1 (SDF-1)<\/jats:italic> is important for the regenerative research in ototoxicity. Mice models with aminoglycoside ototoxicity have been widely used to study the regeneration capacity of MSCs in repair of cochlear injury. We developed a mouse model with maximal increase in SDF-1<\/jats:italic> levels in the inner ear, according to the \u201cone-shot\u201d doses of kanamycin and furosemide. Methods<\/jats:italic>. C57BL\/6 mice had kanamycin (420, 550, and 600\u2009mg\/kg) dissolved in PBS, followed by an intraperitoneal injection of furosemide (130\u2009mg\/kg). The injuries of inner ear were measured with hearing thresholds, histology, and outer hair cell counts at 0, 3, 5, 7, 10, and 14 days before the sacrifice. The levels of SDF-1<\/jats:italic> in the inner ear were tested by real-time RT-PCR and immunohistochemistry. Results<\/jats:italic>. There were a significant reduction in hearing thresholds and a maximal increase of SDF-1<\/jats:italic> levels in the furosemide 130\u2009mg\/kg + kanamycin 550\u2009mg\/kg group, but severe hearing deterioration over time was observed in the furosemide 130\u2009mg\/kg + kanamycin 600\u2009mg\/kg group and four mice were dead. SDF-1<\/jats:italic> was detected mostly in the stria vascularis and organ of Corti showing the highest increase in expression. Conclusion<\/jats:italic>. We observed optimal induction of the stem cell homing factor in the newly generated aminoglycoside-induced ototoxicity mouse model using a \u201cone-shot\u201d protocol. This study regarding high SDF-1<\/jats:italic> levels in our mouse model of ototoxicity would play a major role in the development of therapeutic agents using MSC homing.<\/jats:p>","DOI":"10.1155\/2017\/4630241","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T18:42:43Z","timestamp":1513881763000},"page":"1-9","source":"Crossref","is-referenced-by-count":11,"title":["A Simple Model for Inducing Optimal Increase of SDF-1<\/i> with Aminoglycoside Ototoxicity"],"prefix":"10.1155","volume":"2017","author":[{"given":"Hyun Mi","family":"Ju","sequence":"first","affiliation":[{"name":"Laboratory of Smile Snail, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"},{"name":"Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6045-0295","authenticated-orcid":true,"given":"Sun Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory of Smile Snail, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"},{"name":"Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Sil","family":"Choi","sequence":"additional","affiliation":[{"name":"Laboratory of Smile Snail, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"},{"name":"Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2839-4676","authenticated-orcid":true,"given":"Young Joon","family":"Seo","sequence":"additional","affiliation":[{"name":"Laboratory of Smile Snail, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"},{"name":"Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2015.03.002"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1258\/la.2009.009046"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21729"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00002"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1177\/0194599813519071"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1097\/01.moh.0000245698.62511.3d"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.03.003"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1177\/1084713812440336"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2013.34"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0687"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2007.06610.x"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1172\/JCI67621"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(95)90110-8"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(97)00133-0"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5955(02)00768-2"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1038\/srep08181"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuro.2013.11.002"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060948"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2017.00332"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.7874\/jao.2017.21.2.81"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/4630241.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/4630241.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/4630241.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T18:42:51Z","timestamp":1513881771000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/4630241\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":20,"alternative-id":["4630241","4630241"],"URL":"https:\/\/doi.org\/10.1155\/2017\/4630241","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2017]]}}}