{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:29:45Z","timestamp":1740169785228,"version":"3.37.3"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012046","name":"Vietnam Academy of Science and Technology","doi-asserted-by":"crossref","award":["VAST.CTG.02\/15-16"],"award-info":[{"award-number":["VAST.CTG.02\/15-16"]}],"id":[{"id":"10.13039\/100012046","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Many studies have comprehensively examined the venom of<jats:italic>Ophiophagus hannah<\/jats:italic>snake. Its venom comprises different compounds exhibiting a wide range of pharmacological activities. In this investigation, four peptide fractions (PFs), ranging from 3\u2009kDa to 10\u2009kDa, isolated from the Vietnamese snake venom of<jats:italic>O. hannah<\/jats:italic>were separated by HPLC and investigated for their inhibitory activity on adipogenesis in 3T3-L1 adipocytes. The most effective PF was then further purified, generating two peptides, pOh1 and pOh2. Upon investigation of these two peptides on 3T3-L1 adipocytes, it was revealed that, at 10\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL, pOh2 was able to inhibit the lipid accumulation in 3T3-L1 adipocytes by up to 56%, without affecting cell viability. Furthermore, the pOh2 downregulated the gene expression of important transcription factors C\/EBP-<jats:italic>\u03b1<\/jats:italic>and PPAR-<jats:italic>\u03b3<\/jats:italic>. In addition, aP2 and GPDH adipocyte-specific markers were also significantly reduced compared to untreated differentiated cells. Taken together, pOh2 inhibited the expression of key transcription factors C\/EBP-<jats:italic>\u03b1<\/jats:italic>and PPAR-<jats:italic>\u03b3<\/jats:italic>and their target genes, aP2 and GPDH, thereby blocking the adipocyte differentiation. In conclusion, this novel class of peptide might have potential for in vivo antiobesity effects.<\/jats:p>","DOI":"10.1155\/2017\/4826595","type":"journal-article","created":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T21:14:46Z","timestamp":1490303686000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["Peptide Fraction pOh2 Exerts Antiadipogenic Activity through Inhibition of C\/EBP-<i>\u03b1<\/i>and PPAR-<i>\u03b3<\/i>Expression in 3T3-L1 Adipocytes"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4441-2862","authenticated-orcid":true,"given":"Thi Tuyet Nhung","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Street, Cau Giay District, Hanoi 100000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thi Thu","family":"Ha","sequence":"additional","affiliation":[{"name":"Institute of 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