{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:56:27Z","timestamp":1791824187470,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["STIF201110"],"award-info":[{"award-number":["STIF201110"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["B2012078"],"award-info":[{"award-number":["B2012078"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["2015A030313513"],"award-info":[{"award-number":["2015A030313513"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["81573932"],"award-info":[{"award-number":["81573932"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["81273237"],"award-info":[{"award-number":["81273237"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["81570009"],"award-info":[{"award-number":["81570009"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["STIF201110"],"award-info":[{"award-number":["STIF201110"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["B2012078"],"award-info":[{"award-number":["B2012078"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["2015A030313513"],"award-info":[{"award-number":["2015A030313513"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["81573932"],"award-info":[{"award-number":["81573932"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["81273237"],"award-info":[{"award-number":["81273237"]}]},{"name":"Science and Technology Innovation Fund of Guangdong Medical College","award":["81570009"],"award-info":[{"award-number":["81570009"]}]},{"name":"Provincial Natural Science Foundation of Guangdong Province","award":["STIF201110"],"award-info":[{"award-number":["STIF201110"]}]},{"name":"Provincial Natural Science Foundation of Guangdong Province","award":["B2012078"],"award-info":[{"award-number":["B2012078"]}]},{"name":"Provincial Natural Science Foundation of Guangdong Province","award":["2015A030313513"],"award-info":[{"award-number":["2015A030313513"]}]},{"name":"Provincial Natural Science Foundation of Guangdong Province","award":["81573932"],"award-info":[{"award-number":["81573932"]}]},{"name":"Provincial Natural Science Foundation of Guangdong Province","award":["81273237"],"award-info":[{"award-number":["81273237"]}]},{"name":"Provincial Natural Science Foundation of Guangdong Province","award":["81570009"],"award-info":[{"award-number":["81570009"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["STIF201110"],"award-info":[{"award-number":["STIF201110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["B2012078"],"award-info":[{"award-number":["B2012078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2015A030313513"],"award-info":[{"award-number":["2015A030313513"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573932"],"award-info":[{"award-number":["81573932"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273237"],"award-info":[{"award-number":["81273237"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570009"],"award-info":[{"award-number":["81570009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["STIF201110"],"award-info":[{"award-number":["STIF201110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["B2012078"],"award-info":[{"award-number":["B2012078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2015A030313513"],"award-info":[{"award-number":["2015A030313513"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573932"],"award-info":[{"award-number":["81573932"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273237"],"award-info":[{"award-number":["81273237"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570009"],"award-info":[{"award-number":["81570009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["STIF201110"],"award-info":[{"award-number":["STIF201110"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["B2012078"],"award-info":[{"award-number":["B2012078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2015A030313513"],"award-info":[{"award-number":["2015A030313513"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573932"],"award-info":[{"award-number":["81573932"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273237"],"award-info":[{"award-number":["81273237"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570009"],"award-info":[{"award-number":["81570009"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Colitis is a major form of inflammatory bowel disease which involved mucosal immune dysfunction. Aloperine is an alkaloid isolated from the shrub <jats:italic>Sophora alopecuroides<\/jats:italic> L. and has been recognized as an effective treatment for inflammatory and allergic diseases. The present study aimed to examine the molecular mechanisms underlying aloperine-mediated colitis protection. We found that aloperine treatment improved colitis induced by dextran sodium sulfate (DSS) based on body weight, disease activity index, colonic length, and spleen index. Aloperine also effectively attenuated DSS-induced intestinal inflammation based on the pathological score and myeloperoxidase expression and activity in colon tissues. In addition, aloperine regulated T-cell proportions and promoted Foxp3 expression in the spleens and mesenteric lymph nodes of DSS-induced colitis mice and in the spleens of the <jats:italic>Foxp3<\/jats:italic><jats:sup>GFP<\/jats:sup> mice. Aloperine inhibited Jurkat and mouse na\u00efve T-cell apoptosis. Furthermore, aloperine inhibited PI3K\/Akt\/mTOR signaling and upregulated PP2A expression in the DSS-induced colitis mice and in Jurkat cells, but LB-100 (PP2A inhibitor) resulted in an elevated Akt activity in Jurkat cells, activated T-cells, and human splenic mononuclear cells. Aloperine inhibited T-cell and lymphocyte proliferation, but LB-100 reverse these effects. In conclusion, aloperine regulates inflammatory responses in colitis by inhibiting the PI3K\/Akt\/mTOR signaling in a PP2A-dependent manner.<\/jats:p>","DOI":"10.1155\/2017\/5706152","type":"journal-article","created":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T17:02:40Z","timestamp":1505840560000},"page":"1-14","source":"Crossref","is-referenced-by-count":57,"title":["Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K\/Akt\/mTOR Signaling Suppression"],"prefix":"10.1155","volume":"2017","author":[{"given":"Xiaoxia","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China"},{"name":"The Center for Biomedical Research, Key Laboratory of Organ Transplantation, Ministry of Education, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China"},{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9342-0001","authenticated-orcid":true,"given":"Fei","family":"Sun","sequence":"additional","affiliation":[{"name":"The Center for Biomedical Research, Key Laboratory of Organ Transplantation, Ministry of Education, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3772-6692","authenticated-orcid":true,"given":"Faxi","family":"Wang","sequence":"additional","affiliation":[{"name":"The Center for Biomedical Research, Key Laboratory of Organ Transplantation, Ministry of Education, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3580-8910","authenticated-orcid":true,"given":"Junai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China"},{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China"},{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3534-7480","authenticated-orcid":true,"given":"Jixin","family":"Zhong","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute, Case Western Reserve University, 2103 Cornell Road, Cleveland, OH 44106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0307-207X","authenticated-orcid":true,"given":"Tiantian","family":"Yue","sequence":"additional","affiliation":[{"name":"The Center for Biomedical Research, Key Laboratory of Organ Transplantation, Ministry of Education, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6850-9725","authenticated-orcid":true,"given":"Xuebao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China"},{"name":"Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3523-7556","authenticated-orcid":true,"given":"Jun-Fa","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Institute of Laboratory Medicine, Guangdong Medical University, No. 1 Xincheng Road, Dongguan 523808, China"},{"name":"Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, No. 1 Xincheng Road, Dongguan 523808, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong-Yi","family":"Wang","sequence":"additional","affiliation":[{"name":"The Center for Biomedical Research, Key Laboratory of Organ Transplantation, Ministry of Education, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","year":"2000"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-014-0288-2"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.42"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01018.x"},{"issue":"7090","key":"5","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/nature04753","volume":"441","year":"2006","journal-title":"Nature"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001454"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.07.031"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3077"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/nri3198"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2014.1718"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2015.00056"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.08.025"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.02.013"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1021\/jf103581r"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-015-0120-x"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X1000783X"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemlett.5b00339"},{"issue":"7123","key":"18","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1038\/nature05351","volume":"445","year":"2007","journal-title":"Nature"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.160"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.18632\/genesandcancer.128"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.02.025"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1631\/jzus.2007.B0632"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2014.10.011"},{"issue":"12","key":"24","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1046\/j.1440-1746.2002.02868.x","volume":"17","year":"2002","journal-title":"Journal of Gastroenterology and Hepatology"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-1288-1"},{"issue":"4","key":"26","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1084\/jem.194.4.519","volume":"194","year":"2001","journal-title":"The Journal of Experimental Medicine"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.05.035"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2016.36"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.966408"},{"issue":"3","key":"30","first-page":"2627","volume":"8","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"31","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.sjpain.2015.04.001","volume":"8","year":"2015","journal-title":"Scandinavian Journal of Pain"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2010.03288.x"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1520393112"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4063"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3390"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2016.01.009"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1042\/BST20160166"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00007.2016"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3405"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90296.2008"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01152.x"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000098"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1038\/nri2546"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2015.10.001"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.08.012"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-2623"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.05.012"},{"issue":"1","key":"48","first-page":"19","volume":"2","year":"2010","journal-title":"American Journal of Translational Research"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/5706152.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/5706152.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/5706152.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,9,19]],"date-time":"2017-09-19T17:02:54Z","timestamp":1505840574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2017\/5706152\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":48,"alternative-id":["5706152","5706152"],"URL":"https:\/\/doi.org\/10.1155\/2017\/5706152","relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}