{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T21:47:57Z","timestamp":1779313677587,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>miR-135a-5p was reported to play a crucial role in the protective effects of hydrogen sulfide against Parkinson\u2019s disease (PD) by targeting rho-associated protein kinase 2 (ROCK2). However, the role of another member of miR-135 family (miR-135b) and the underlying mechanism in PD are still unclear. qRT-PCR and western blot showed that miR-135 was downregulated and glycogen synthase kinase 3<jats:italic>\u03b2<\/jats:italic>(GSK3<jats:italic>\u03b2<\/jats:italic>) was upregulated at mRNA and protein levels in MPP<jats:sup>+<\/jats:sup>-intoxicated SH-SY5Y cells in a dose- and time-dependent manner. MTT, TUNEL, and ELISA assays revealed that miR-135b overexpression significantly promoted cell proliferation and inhibited apoptosis and production of TNF-<jats:italic>\u03b1<\/jats:italic>and IL-1<jats:italic>\u03b2<\/jats:italic>in SH-SY5Y cells in the presence of MPP<jats:sup>+<\/jats:sup>. Luciferase reporter assay demonstrated that GSK3<jats:italic>\u03b2<\/jats:italic>was a direct target of miR-135b. Moreover, sodium nitroprusside (SNP), a GSK3<jats:italic>\u03b2<\/jats:italic>activator, dramatically reversed the effects of miR-135b upregulation on cell proliferation, apoptosis, and inflammatory cytokine production in MPP<jats:sup>+<\/jats:sup>-intoxicated SH-SY5Y cells. Taken together, miR-135b exerts a protective role via promotion of proliferation and suppression of apoptosis and neuroinflammation by targeting GSK3<jats:italic>\u03b2<\/jats:italic>in MPP<jats:sup>+<\/jats:sup>-intoxicated SH-SY5Y cells, providing a potential therapeutic target for the treatment of PD.<\/jats:p>","DOI":"10.1155\/2017\/5806146","type":"journal-article","created":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T21:01:12Z","timestamp":1492549272000},"page":"1-10","source":"Crossref","is-referenced-by-count":36,"title":["miR-135b Plays a Neuroprotective Role by Targeting GSK3\u03b2 in MPP<sup>+<\/sup>-Intoxicated SH-SY5Y Cells"],"prefix":"10.1155","volume":"2017","author":[{"given":"Jianlei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurology, Huaihe Hospital of Henan University, Kaifeng 475000, 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