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However, whether and how EA act on hippocampal neurogenesis following traumatic brain injury (TBI) remains poorly understood. This study aims to investigate the effect of EA on hippocampal neurogenesis and neurological functions, as well as its underlying association with toll-like receptor 4 (TLR4) signaling in TBI mice. BrdU\/NeuN immunofluorescence was performed to label newborn neurons in the hippocampus after EA treatment. Water maze test and neurological severity score were used to evaluate neurological function posttrauma. The hippocampal level of TLR4 and downstream molecules and inflammatory cytokines were, respectively, detected by Western blot and enzyme-linked immunosorbent assay. EA enhanced hippocampal neurogenesis and inhibited TLR4 expression at 21, 28, and 35 days after TBI, but the beneficial effects of EA on posttraumatic neurogenesis and neurological functions were attenuated by lipopolysaccharide-induced TLR4 activation. In addition, EA exerted an inhibitory effect on both TLR4\/Myd88\/NF-<jats:italic>\u03ba<\/jats:italic>B and TLR4\/TRIF\/NF-<jats:italic>\u03ba<\/jats:italic>B pathways, as well as the inflammatory cytokine expression in the hippocampus following TBI. In conclusion, EA promoted hippocampal neurogenesis and neurological recovery through inhibition of TLR4 signaling pathway posttrauma, which may be a potential approach to improve the outcome of TBI.<\/jats:p>","DOI":"10.1155\/2017\/5841814","type":"journal-article","created":{"date-parts":[[2017,8,7]],"date-time":"2017-08-07T21:02:08Z","timestamp":1502139728000},"page":"1-13","source":"Crossref","is-referenced-by-count":15,"title":["Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5645-910X","authenticated-orcid":true,"given":"Yuqin","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi\u2019an 710032, China"},{"name":"Department of Neurosurgery, PLA 163rd Hospital (Second Affiliated Hospital of Hunan Normal University), 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