{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T20:46:41Z","timestamp":1762980401560,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["15-54-78077","16-34-01342","15-29-01228"],"award-info":[{"award-number":["15-54-78077","16-34-01342","15-29-01228"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["15-54-78077","16-34-01342","15-29-01228"],"award-info":[{"award-number":["15-54-78077","16-34-01342","15-29-01228"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["15-54-78077","16-34-01342","15-29-01228"],"award-info":[{"award-number":["15-54-78077","16-34-01342","15-29-01228"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002674","name":"Russian Academy of Sciences","doi-asserted-by":"publisher","award":["15-54-78077","16-34-01342","15-29-01228"],"award-info":[{"award-number":["15-54-78077","16-34-01342","15-29-01228"]}],"id":[{"id":"10.13039\/501100002674","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Erythrocytes (RBCs) loaded with alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALD) can metabolize plasma ethanol and acetaldehyde but with low efficiency. We investigated the rate-limiting factors in ethanol oxidation by these enzymes loaded into RBCs. Mathematical modeling and in vitro experiments on human RBCs loaded simultaneously with ADH and ALD (by hypoosmotic dialysis) were performed. The simulation showed that the rate of nicotinamide-adenine dinucleotide (NAD<jats:sup>+<\/jats:sup>) generation in RBC glycolysis, but not the activities of the loaded enzymes, is the rate-limiting step in external ethanol oxidation. The rate of oxidation could be increased if RBCs are supplemented by NAD<jats:sup>+<\/jats:sup>and pyruvate. Our experimental data verified this theoretical conclusion. RBCs loaded with the complete system of ADH, ALD, NAD<jats:sup>+<\/jats:sup>, and pyruvate metabolized ethanol 20\u201340 times faster than reported in previous studies. The one-step procedure of hypoosmotic dialysis is the optimal method to encapsulate ADH and ALD in RBCs after cell recovery, encapsulation yield, osmotic resistance, and RBC-indexes. Consequently, transfusion of the RBCs loaded with the complete metabolic system, including ADH, ALD, pyruvate, and NAD<jats:sup>+<\/jats:sup>in the patients with alcohol intoxication, may be a promising method for rapid detoxification of blood alcohol based on metabolism.<\/jats:p>","DOI":"10.1155\/2017\/5849593","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T21:08:40Z","timestamp":1496264920000},"page":"1-14","source":"Crossref","is-referenced-by-count":11,"title":["Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study"],"prefix":"10.1155","volume":"2017","author":[{"given":"Yuliya G.","family":"Alexandrovich","sequence":"first","affiliation":[{"name":"Laboratory of Biophysics and Physiology of the Cell, Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Kosygin Street 4, Moscow 119334, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3293-652X","authenticated-orcid":true,"given":"Elena A.","family":"Kosenko","sequence":"additional","affiliation":[{"name":"Laboratory of Modeling and Bioinformatics, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pyshchino, Moscow Region 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5948-3444","authenticated-orcid":true,"given":"Elena I.","family":"Sinauridze","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics and Physiology of the Cell, Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Kosygin Street 4, Moscow 119334, Russia"},{"name":"Laboratory of Biophysics, National Scientific and Practical Centre of Pediatric Hematology, Oncology and Immunology Named after Dmitry Rogachev, Russian Ministry of Health, Samory Mashela Street 1, GSP-7, Moscow 117198, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2930-8768","authenticated-orcid":true,"given":"Sergey I.","family":"Obydennyi","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics and Physiology of the Cell, Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Kosygin Street 4, Moscow 119334, Russia"},{"name":"Laboratory of Cell Hemostasis and Thrombosis, National Scientific and Practical Centre of Pediatric Hematology, Oncology and Immunology Named after Dmitry Rogachev, Russian Ministry of Health, Samory Mashela Street 1, GSP-7, Moscow 117198, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor I.","family":"Kireev","sequence":"additional","affiliation":[{"name":"Department of Electron Microscopy, Moscow State University, Belozersky Institute of Physico-Chemical Biology, Leninskie Gory 1, Building 40, Moscow 119992, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6668-0948","authenticated-orcid":true,"given":"Fazoil I.","family":"Ataullakhanov","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics and Physiology of the Cell, Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, Kosygin Street 4, Moscow 119334, Russia"},{"name":"Laboratory of Biophysics, National Scientific and Practical Centre of Pediatric Hematology, Oncology and Immunology Named after Dmitry Rogachev, Russian Ministry of Health, Samory Mashela Street 1, GSP-7, Moscow 117198, Russia"},{"name":"Faculty of Physics, Moscow State University, Leninskie Gory 1, Building 2, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuriy G.","family":"Kaminsky","sequence":"additional","affiliation":[{"name":"Laboratory of Modeling and Bioinformatics, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Street 3, Pyshchino, Moscow Region 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"volume":"100","year":"2017","key":"1"},{"issue":"1","key":"2","first-page":"5","volume":"30","year":"2007","journal-title":"Alcohol Research & Health"},{"issue":"51","key":"3","first-page":"157","year":"1985","journal-title":"Bibliotheca Haematologica"},{"year":"1987","key":"4"},{"issue":"1","key":"5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1080\/713840329","volume":"10","year":"2003","journal-title":"Drug Delivery: Journal of Delivery and Targeting of Therapeutic Agents"},{"doi-asserted-by":"publisher","key":"6","DOI":"10.3324\/haematol.10770"},{"issue":"7","key":"7","first-page":"91","volume":"80","year":"2008","journal-title":"Terapevticheskii Arkhiv"},{"doi-asserted-by":"publisher","key":"8","DOI":"10.1517\/14712598.2012.631909"},{"doi-asserted-by":"publisher","key":"9","DOI":"10.1172\/JCI108129"},{"doi-asserted-by":"publisher","key":"10","DOI":"10.1007\/s002280050051"},{"doi-asserted-by":"publisher","key":"11","DOI":"10.1111\/j.1600-0609.2007.00927.x"},{"doi-asserted-by":"publisher","key":"12","DOI":"10.1016\/S0024-3205(01)00991-2"},{"doi-asserted-by":"publisher","key":"13","DOI":"10.1016\/S0304-4165(98)00085-3"},{"doi-asserted-by":"publisher","key":"14","DOI":"10.1007\/s10517-012-1668-x"},{"issue":"8","key":"28","doi-asserted-by":"crossref","first-page":"3754","DOI":"10.1016\/S0021-9258(18)61419-X","volume":"262","year":"1987","journal-title":"Journal of Biological Chemistry"},{"issue":"11","key":"29","doi-asserted-by":"crossref","first-page":"7792","DOI":"10.1016\/S0021-9258(18)53028-3","volume":"268","year":"1993","journal-title":"Journal of Biological Chemistry"},{"doi-asserted-by":"publisher","key":"30","DOI":"10.1042\/bj1730787"},{"doi-asserted-by":"publisher","key":"31","DOI":"10.1016\/S0076-6879(75)41079-5"},{"doi-asserted-by":"publisher","key":"32","DOI":"10.1042\/bj3630769"},{"issue":"4","key":"33","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/S0021-9258(19)81726-X","volume":"243","year":"1968","journal-title":"The Journal of Biological Chemistry"},{"doi-asserted-by":"publisher","key":"25","DOI":"10.1016\/S0304-4165(99)00218-4"},{"issue":"5","key":"34","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1016\/S0021-9258(19)83760-2","volume":"237","year":"1962","journal-title":"The Journal of Biological Chemistry"},{"doi-asserted-by":"publisher","key":"15","DOI":"10.3109\/10731198809117275"},{"doi-asserted-by":"publisher","key":"16","DOI":"10.1139\/o08-134"},{"doi-asserted-by":"publisher","key":"17","DOI":"10.1080\/10715760601161452"},{"year":"2017","key":"18"},{"doi-asserted-by":"publisher","key":"19","DOI":"10.1093\/toxsci\/kfq031"},{"year":"1975","key":"20"},{"doi-asserted-by":"publisher","key":"21","DOI":"10.1002\/(SICI)1096-8652(199806)58:2<117::AID-AJH5>3.0.CO;2-V"},{"doi-asserted-by":"publisher","key":"22","DOI":"10.3748\/wjg.15.5674"},{"issue":"6","key":"23","first-page":"404","volume":"18","year":"1997","journal-title":"Pediatric Dentistry"},{"doi-asserted-by":"publisher","key":"24","DOI":"10.1111\/j.1530-0277.2002.tb02543.x"},{"doi-asserted-by":"publisher","key":"26","DOI":"10.1186\/1752-0509-4-109"},{"doi-asserted-by":"publisher","key":"27","DOI":"10.1093\/alcalc\/agh168"},{"doi-asserted-by":"publisher","key":"35","DOI":"10.1042\/cs0960171"},{"doi-asserted-by":"publisher","key":"36","DOI":"10.1016\/S0024-3205(99)00178-2"},{"doi-asserted-by":"publisher","key":"37","DOI":"10.1093\/oxfordjournals.alcalc.a045059"},{"doi-asserted-by":"publisher","key":"38","DOI":"10.1016\/S0024-3205(99)00546-9"},{"volume-title":"Biochemical properties of alcohol dehydrogenase and glutamate dehydrogenase encapsulated into human erythrocytes by a hypotonic-dialysis procedure","year":"1997","first-page":"101","key":"39"},{"doi-asserted-by":"publisher","key":"40","DOI":"10.1080\/00039896.1975.10666763"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/5849593.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/5849593.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/5849593.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:49:34Z","timestamp":1659055774000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/5849593\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":40,"alternative-id":["5849593","5849593"],"URL":"https:\/\/doi.org\/10.1155\/2017\/5849593","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2017]]}}}