{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:19:14Z","timestamp":1790432354952,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,29]],"date-time":"2017-10-29T00:00:00Z","timestamp":1509235200000},"content-version":"vor","delay-in-days":301,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL88940"],"award-info":[{"award-number":["HL88940"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL77448"],"award-info":[{"award-number":["HL77448"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470557"],"award-info":[{"award-number":["81470557"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30500198"],"award-info":[{"award-number":["30500198"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30821001"],"award-info":[{"award-number":["30821001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Reversible myocardial ischemia\/reperfusion (I\/R) or ischemic preconditioning (IPC) is associated with an immediate genomic response; IPC\u2010induced immediate early genes are associated with reduced infarct size. Because the immediate early response gene X\u20101 (IEX\u20101) plays a central role in cell apoptosis, we examine whether IEX\u20101 exerts protective effects against I\/R injury. We found that the IEX\u20101 mRNA level was increased in the IPC\u2010imposed rat heart. However, it was downregulated in the I\/R rat heart, which was prevented by in situ IPC. When IEX\u20101 was knocked down, the protective effects imposed by IPC were lessened. Local gene delivery of Ad\u2010IEX\u20101 to the left ventricle greatly diminished cardiac infarct size and improved systolic functions of I\/R hearts in rats. In contrast, knocking down IEX\u20101 expression exacerbates myocardial infarction. Overexpression of IEX\u20101 in neonatal rat cardiomyocytes significantly reduced hypoxia\u2010reoxygenation\u2010induced intracellular and mitochondrial ROS accumulation and cell apoptosis. Furthermore, IPC\u2010induced phosphorylation and particle translocation of PKC\u03b5 were impaired by knocking down IEX\u20101 in vivo, and overexpressing IEX\u20101 showed similar cardioprotection imposed by IPC. Our results demonstrate that IPC increases IEX\u20101 expression, which may promote phosphorylation and particle translocation of PKC\u03b5 and thus reduce intracellular ROS accumulation. These beneficial effects reduce cardiomyocyte apoptosis and necrosis to alleviate cardiac infarction.<\/jats:p>","DOI":"10.1155\/2017\/6109061","type":"journal-article","created":{"date-parts":[[2017,10,29]],"date-time":"2017-10-29T23:31:48Z","timestamp":1509319908000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Immediate Early Response Gene X\u20101 (IEX\u20101) Mediates Ischemic Preconditioning\u2010Induced Cardioprotection in Rats"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4184-0797","authenticated-orcid":false,"given":"Ming-Jiang","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijuan","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John Y.-J.","family":"Shyy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1138-871X","authenticated-orcid":false,"given":"Xian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,29]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112160"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017538115617"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2007.05.008"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00059-008-3101-9"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.02.015"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2003.09.023"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0003-4975(97)00272-5"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/27.4.578"},{"key":"e_1_2_7_9_2","first-page":"159","article-title":"Cardiac gene expression after brief coronary occlusion","volume":"84","author":"Zimmermann R.","year":"1995","journal-title":"Zeitschrift f\u00fcr Kardiologie"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1021688600370"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf488"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600054200"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204061"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(01)02344-4"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204965"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-3047"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18040723"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600980"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609712200"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7254.2005.00139.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000138946.29375.49"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.103.21.2617"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(92)90457-x"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf00054745"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46660-9"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.77.4.314"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.88.3.1264"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2007.01.025"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2005.03.050"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.022326699"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1159\/000077253"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/14.suppl_G.25"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.96.2.614"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.13.6770"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.805242"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.6.H2240"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00711.2002"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00533-9"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0008-6363(02)00455-8"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19906"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000087542.26971.D4"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0351052"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000245106.80628.d3"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000069215.36389.8D"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050757"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00434.2001"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12903"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000012922.40318.05"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000103635.38096.2F"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00306.2005"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2017\/6109061.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2017\/6109061.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2017\/6109061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T04:31:20Z","timestamp":1722918680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2017\/6109061"}},"subtitle":[],"editor":[{"given":"Aditya","family":"Sen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1155\/2017\/6109061"],"URL":"https:\/\/doi.org\/10.1155\/2017\/6109061","archive":["Portico"],"relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"2017-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6109061"}}