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Low-virulence anaerobic bacteria, especially the<jats:italic>Propionibacterium acnes (P. acnes)<\/jats:italic>, have been thought to be a new pathogeny for a series of disc diseases. However, until now, there has been no histological evidence to confirm this link. The purpose of this study was to confirm the presence of<jats:italic>P. acnes<\/jats:italic>in nonpyogenic intervertebral discs via histological observation.<jats:italic>Method<\/jats:italic>. Degenerated intervertebral discs were harvested from 76 patients with low back pain and\/or sciatica but without any symptoms of discitis or spondylodiscitis. The samples were cultured under anaerobic conditions and then examined using 16S rDNA PCR to screen for<jats:italic>P. acnes<\/jats:italic>. Samples found to be positive for<jats:italic>P. acnes<\/jats:italic>were stained with hematoxylin-eosin (HE) and modified Brown-Brenn staining and observed under a microscope.<jats:italic>Results<\/jats:italic>. Here, 16 intervertebral discs were found to be positive for<jats:italic>P. acnes<\/jats:italic>via 16S rDNA PCR and the prevalence was 21.05% (16\/76). Among them, 7 samples had visible microbes stained with HE and modified Brown-Brenn staining. Morphological examination showed the bacteria to be Gram-positive and rod-shaped, so they were considered<jats:italic>P. acnes<\/jats:italic>.<jats:italic>Conclusion<\/jats:italic>.<jats:italic>P. acnes<\/jats:italic>is capable of colonizing some degenerated intervertebral discs without causing discitis, and its presence could be further confirmed by histological evidence. Targeting these bacteria may be a promising therapy method for some disc diseases.<\/jats:p>","DOI":"10.1155\/2017\/6192935","type":"journal-article","created":{"date-parts":[[2017,3,19]],"date-time":"2017-03-19T21:01:26Z","timestamp":1489957286000},"page":"1-7","source":"Crossref","is-referenced-by-count":27,"title":["Histological Identification of<i>Propionibacterium acnes<\/i>in Nonpyogenic Degenerated Intervertebral Discs"],"prefix":"10.1155","volume":"2017","author":[{"given":"Ye","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"},{"name":"Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 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