{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:00:39Z","timestamp":1792674039427,"version":"4.5.0"},"reference-count":88,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lithuanian University of Health Science","award":["V\u20131238"],"award-info":[{"award-number":["V\u20131238"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Literature data support evidences that glioblastoma (GBM) patients experience prolonged survival due to sodium valproate (NaVP) treatment. The study assessed the human GBM cell U87 xenograft studied in the chicken embryo chorioallantoic membrane (CAM) model evaluating NaVP effect on tumor. Three groups of tumors (each<jats:italic>n<\/jats:italic>= 10) were studied: nontreated, treated with 4\u2009mM, and treated with 8\u2009mM of NaVP. The majority of tumors without NaVP treatment during tumor growth destroyed the chorionic epithelium, invaded the mesenchyme, and induced angiogenesis. Incidence of tumor formation on CAM without invasion into the mesenchyme was higher when U87 cells were treated with NaVP; the effect significantly increased with NaVP concentration. Treatment with 8\u2009mM of NaVP did not show clear dynamics of tumor growth during 5 days; at the same time, the angiogenesis failed. With a strong staining of EZH2, p53 in tumors without NaVP treatment was found, and NaVP significantly decreased the expression of EZH2- and p53-positive cells; the effect was significantly higher at its 8\u2009mM concentration. NaVP has a function in blocking the growth, invasion, and angiogenesis of tumor in the CAM model; tumor growth interferes with EZH2 and p53 molecular pathways, supporting the NaVP potential in GBM therapy.<\/jats:p>","DOI":"10.1155\/2017\/6326053","type":"journal-article","created":{"date-parts":[[2017,5,31]],"date-time":"2017-05-31T21:11:21Z","timestamp":1496265081000},"page":"1-12","source":"Crossref","is-referenced-by-count":8,"title":["The Effect of Sodium Valproate on the Glioblastoma U87 Cell Line Tumor Development on the Chicken Embryo Chorioallantoic Membrane and on EZH2 and p53 Expression"],"prefix":"10.1155","volume":"2017","author":[{"given":"Dovil\u0117","family":"Kavaliauskait\u0117","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Lithuanian University of Health Sciences, Mickeviciaus Str., LT-44307 Kaunas, 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