{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T12:58:04Z","timestamp":1771592284964,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81601672"],"award-info":[{"award-number":["81601672"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["1191320114"],"award-info":[{"award-number":["1191320114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"the National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["2013KCJ-23"],"award-info":[{"award-number":["2013KCJ-23"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Fundamental Research Funds for the Central Universities","award":["81601672"],"award-info":[{"award-number":["81601672"]}]},{"name":"the Fundamental Research Funds for the Central Universities","award":["1191320114"],"award-info":[{"award-number":["1191320114"]}]},{"name":"the Fundamental Research Funds for the Central Universities","award":["2013KCJ-23"],"award-info":[{"award-number":["2013KCJ-23"]}]},{"name":"the Project of Innovative Research Team for Key Science and Technology in Shaanxi province","award":["81601672"],"award-info":[{"award-number":["81601672"]}]},{"name":"the Project of Innovative Research Team for Key Science and Technology in Shaanxi province","award":["1191320114"],"award-info":[{"award-number":["1191320114"]}]},{"name":"the Project of Innovative Research Team for Key Science and Technology in Shaanxi province","award":["2013KCJ-23"],"award-info":[{"award-number":["2013KCJ-23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection with a high mortality. 5-Hydroxytryptamine (5-HT) is an important regulatory factor in inflammation. The aim of this study is to investigate the role of 5-HT on cecal ligation and puncture- (CLP-) induced sepsis in the mouse model. CLP was performed on C57B\/6 wild-type (WT) mice and <jats:italic>tryptophan hydroxylase 1<\/jats:italic> (<jats:italic>TPH1<\/jats:italic>) knockout (KO) mice. The results showed that the 5-HT-sufficient group mice had a significantly lower survival rate than the 5-HT-deficient group in CLP-induced sepsis and septic shock. The KO-CLP sepsis group received a lower clinical score than the WT-CLP sepsis group. Meanwhile, the body temperature of mice in the KO-CLP sepsis group was higher than that in the WT-CLP sepsis group and was much closer to the normal body temperature 24\u2009hours after CLP. The tissue histopathology analysis revealed that 5-HT markedly exacerbated histological damages in the peritoneum, lung, liver, kidney, intestinal tissue, and heart in sepsis. Moreover, significant lower levels of TNF-<jats:italic>\u03b1<\/jats:italic>, IL-6, bacterial loads, MPO, and ROS were discovered in the KO-CLP sepsis group in contrast to the WT-CLP sepsis group. In conclusion, 5-HT drives mortality and exacerbates organ dysfunction by promoting serum cytokines and bacterial loads as well as facilitating oxidative stress in the process of sepsis.<\/jats:p>","DOI":"10.1155\/2017\/6374283","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T17:02:47Z","timestamp":1497373367000},"page":"1-12","source":"Crossref","is-referenced-by-count":32,"title":["5-HT Drives Mortality in Sepsis Induced by Cecal Ligation and Puncture in Mice"],"prefix":"10.1155","volume":"2017","author":[{"given":"Jingyao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"},{"name":"Department of Surgical Intensive Care Unit, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbin","family":"Bi","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7318-1004","authenticated-orcid":true,"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"},{"name":"Department of Surgical Intensive Care Unit, The First Affiliated Hospital of Xi\u2019an Jiaotong University, Xi\u2019an, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0287"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.0288"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-017-4683-6"},{"issue":"4","key":"4","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1615\/CritRevImmunol.2015015461","volume":"35","year":"2015","journal-title":"Critical Reviews in Immunology"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.5301\/uro.5000113"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1284043"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.surge.2012.03.003"},{"issue":"1","key":"8","first-page":"83","volume":"41","year":"2003","journal-title":"Romanian Journal of Internal Medicine"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2013.08.001"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2014.07.005"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7613.144922"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2008.00944.x"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2233056100"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.4306\/pi.2010.7.1.60"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1126\/science.1078197"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12430"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.10.031"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2015.0258"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-07320-0_9"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000290"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/srep08098"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.047"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2013.105"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200911-1757OC"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2007.05.019"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-437392"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1007\/BF03022837"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1038\/srep22747"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1038\/nm1780"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1126\/science.1123842"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4268"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.01.002"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000318"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1177\/0148607195019004319"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.118870"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1159\/000206143"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200102000-00051"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5359768"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.5482\/HAMO-14-11-0073"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20120408"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1080\/00365540310015935"},{"issue":"2","key":"42","first-page":"243","volume":"114","year":"1993","journal-title":"Surgery"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1159\/000316286"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aes577"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aer093"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0322-6"},{"issue":"12","key":"47","first-page":"1257","volume":"18","year":"2015","journal-title":"Iranian Journal of Basic Medical Sciences"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.06.023"},{"issue":"10","key":"49","first-page":"1472","volume":"35","year":"2015","journal-title":"Nan Fang Yi Ke da Xue Xue Bao"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-015-0296-9"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/6374283.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/6374283.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2017\/6374283.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T17:02:57Z","timestamp":1497373377000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/mi\/2017\/6374283\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":50,"alternative-id":["6374283","6374283"],"URL":"https:\/\/doi.org\/10.1155\/2017\/6374283","relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}