{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:52:53Z","timestamp":1791496373665,"version":"4.3.4"},"reference-count":57,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY014768"],"award-info":[{"award-number":["EY014768"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY014768"],"award-info":[{"award-number":["EY014768"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"TissueTech, Inc., Miami, FL","award":["EY014768"],"award-info":[{"award-number":["EY014768"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Ophthalmology"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. To evaluate the efficacy of self-retained cryopreserved amniotic membrane (CAM) in promoting corneal nerve regeneration and improving corneal sensitivity in dry eye disease (DED).<jats:italic>Methods<\/jats:italic>. In this prospective randomized clinical trial, subjects with DED were randomized to receive CAM (study group) or conventional maximum treatment (control). Changes in signs and symptoms, corneal sensitivity, topography, and in vivo confocal microscopy (IVCM) were evaluated at baseline, 1 month, and 3 months.<jats:italic>Results<\/jats:italic>. Twenty subjects (age 66.9\u2009\u00b1\u20098.9) were enrolled and 17 completed all follow-up visits. Signs and symptoms were significantly improved in the study group yet remained constant in the control. IVCM showed a significant increase in corneal nerve density in the study group (12,241\u2009\u00b1\u20095083\u2009<jats:italic>\u03bc<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup>at baseline, 16,364\u2009\u00b1\u20093734\u2009<jats:italic>\u03bc<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup>at 1 month, and 18,827\u2009\u00b1\u20095453\u2009<jats:italic>\u03bc<\/jats:italic>m\/mm<jats:sup>2<\/jats:sup>at 3 months,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.015<\/mml:mn><\/mml:math>) but was unchanged in the control. This improvement was accompanied with a significant increase in corneal sensitivity (3.25\u2009\u00b1\u20090.6\u2009cm at baseline, 5.2\u2009\u00b1\u20090.5\u2009cm at 1 month, and 5.6\u2009\u00b1\u20090.4\u2009cm at 3 months,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>) and corneal topography only in the study group.<jats:italic>Conclusions<\/jats:italic>. Self-retained CAM is a promising therapy for corneal nerve regeneration and accelerated recovery of the ocular surface health in patients with DED. The study is registered at clinicaltrials.gov with trial identifier:<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/ct2\/show\/NCT02764814\">NCT02764814<\/jats:ext-link>.<\/jats:p>","DOI":"10.1155\/2017\/6404918","type":"journal-article","created":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T21:03:06Z","timestamp":1502830986000},"page":"1-10","source":"Crossref","is-referenced-by-count":45,"title":["Corneal Nerve Regeneration after Self-Retained Cryopreserved Amniotic Membrane in Dry Eye Disease"],"prefix":"10.1155","volume":"2017","author":[{"given":"Thomas","family":"John","sequence":"first","affiliation":[{"name":"Thomas John Vision Institute, Tinley Park, Cook County, IL, USA"},{"name":"Loyola University at Chicago, Maywood, Chicago, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Tighe","sequence":"additional","affiliation":[{"name":"Ocular Surface Center and TissueTech, Inc., Miami, FL, USA"},{"name":"Florida International University Herbert Wertheim College of Medicine, Miami, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4034-0539","authenticated-orcid":true,"given":"Hosam","family":"Sheha","sequence":"additional","affiliation":[{"name":"Ocular Surface Center and TissueTech, Inc., Miami, FL, USA"},{"name":"Florida International University Herbert Wertheim College of Medicine, Miami, FL, USA"},{"name":"Research Institute of Ophthalmology, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedram","family":"Hamrah","sequence":"additional","affiliation":[{"name":"Boston Image Reading Center, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA"},{"name":"Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeina M.","family":"Salem","sequence":"additional","affiliation":[{"name":"Boston Image Reading Center, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA"},{"name":"Center for Translational Ocular Immunology, Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anny M. S.","family":"Cheng","sequence":"additional","affiliation":[{"name":"Ocular Surface Center and TissueTech, Inc., Miami, FL, USA"},{"name":"Florida International University Herbert Wertheim College of Medicine, Miami, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming X.","family":"Wang","sequence":"additional","affiliation":[{"name":"Wang Vision Institute, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan D.","family":"Rock","sequence":"additional","affiliation":[{"name":"Wang Vision Institute, Nashville, TN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/S1542-0124(12)70081-2"},{"key":"2","first-page":"325","volume-title":"The role of amniotic membrane for managing dry eye disease","year":"2013"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2013.09.002"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1097\/ICU.0b013e3283543b61"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2011.06.014"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-12370"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3629"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2008.03.002"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3109\/08820538.2012.711416"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2012.12.003"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2016.09.004"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2015.07.003"},{"key":"13","first-page":"987","volume":"43","year":"2002","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-013-9401-1"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2008.01.010"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0000000000000712"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(94)90097-3"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431X20155092"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e318294b0c0"},{"key":"20","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1001\/archopht.1990.01070090047037","volume":"108","year":"1990","journal-title":"Archives of Ophthalmology"},{"key":"21","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1097\/00003226-199605000-00002","volume":"15","year":"1996","journal-title":"Cornea"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8708"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-7048"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2015.11.038"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2015.12.007"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2016.07.028"},{"key":"27","doi-asserted-by":"crossref","first-page":"243","DOI":"10.3928\/1081-597X-20040501-08","volume":"20","year":"2004","journal-title":"Journal of Refractive Surgery"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-0762"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1097\/ICL.0000000000000042"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1007\/s10792-014-9969-x"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-17433"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0127"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.4449\/aib.v149i2.1363"},{"key":"34","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.ophtha.2003.10.019","volume":"111","year":"2004","journal-title":"Ophthalmology"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.021881"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.342873"},{"key":"37","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1074\/jbc.274.1.298","volume":"274","year":"1999","journal-title":"The Journal of Biological Chemistry"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R300039200"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-5888"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.479584"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-13094"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-17637"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2015.12.002"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.06.005"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1038\/srep42195"},{"key":"46","first-page":"2906","volume":"41","year":"2000","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"47","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/S0886-3350(00)00467-3","volume":"27","year":"2001","journal-title":"Journal of Cataract & Refractive Surgery"},{"key":"48","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1097\/00003226-200105000-00015","volume":"20","year":"2001","journal-title":"Cornea"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.05.009"},{"key":"50","first-page":"1969","volume":"42","year":"2001","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-3041"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-7617"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.06.022"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-1781"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-8445"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2005.09.033"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.2147\/OPTH.S1067"}],"container-title":["Journal of Ophthalmology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2017\/6404918.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2017\/6404918.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/joph\/2017\/6404918.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T08:14:12Z","timestamp":1602749652000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/joph\/2017\/6404918\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":57,"alternative-id":["6404918","6404918"],"URL":"https:\/\/doi.org\/10.1155\/2017\/6404918","relation":{},"ISSN":["2090-004X","2090-0058"],"issn-type":[{"value":"2090-004X","type":"print"},{"value":"2090-0058","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}