{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:27:43Z","timestamp":1762522063624,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1130093"],"award-info":[{"award-number":["1130093"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["1130093"],"award-info":[{"award-number":["1130093"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>As an alternative brucellosis prevention method, we evaluated the immunogenicity induced by new multivalent DNA vaccines in BALB\/c mice. We constructed the vaccines by fusion of BAB1_0273 and\/or BAB1_0278 open reading frames (ORFs) from genomic island 3 (GI-3) and the<jats:italic>Brucella abortus<\/jats:italic>2308<jats:italic>sodC<\/jats:italic>gene with a link based on prolines and alanines (pV273-<jats:italic>sod<\/jats:italic>, pV278-<jats:italic>sod,<\/jats:italic>and pV273-278-<jats:italic>sod<\/jats:italic>, resp.). Results show that immunization with all tested multivalent DNA vaccines induced a specific humoral and cellular immune response. These novel multivalent vaccines significantly increased the production of IgM, IgG, and IgG2a antibodies as well as IFN-<jats:italic>\u03b3<\/jats:italic>levels and the lymphoproliferative response of splenocytes. Although immunization with these multivalent vaccines induced a typical T-helper 1- (Th1-) dominated immune response, such immunogenicity conferred low protection levels in mice challenged with the<jats:italic>B. abortus<\/jats:italic>2308 pathogenic strain. Our results demonstrated that the expression of BAB1_0273 and\/or BABl_0278 antigens conjugated to SOD protein can polarize mice immunity to a Th1-type phenotype, conferring low levels of protection.<\/jats:p>","DOI":"10.1155\/2017\/6535479","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T21:01:57Z","timestamp":1504818117000},"page":"1-8","source":"Crossref","is-referenced-by-count":13,"title":["Multivalent Fusion DNA Vaccine against<i>Brucella abortus<\/i>"],"prefix":"10.1155","volume":"2017","author":[{"given":"Leonardo","family":"G\u00f3mez","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javiera","family":"Llanos","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilia","family":"Escalona","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darwin","family":"S\u00e1ez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Molina","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Flores","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4358-1015","authenticated-orcid":true,"given":"Angel","family":"O\u00f1ate","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Faculty of Biological Sciences, University of Concepci\u00f3n, Concepci\u00f3n, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034925"},{"issue":"2","key":"2","first-page":"1","volume":"2","year":"2012","journal-title":"Frontiers in cellular and Infection Microbiology"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032514299"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.03.003"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2014.07.007"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001929"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-009-0123-8"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00343-15"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2015.00072"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-6-13"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00255-9"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-015-0199-7"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01350-2"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.2734-2741.2006"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.4161\/hv.18389"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.9.4857-4861.2003"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.09.025"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.09.039"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.06.013"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00125"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)90035-3"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.09.039"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00117"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0450.2000.00402.x"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v26.i5.30"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.20506\/rst.32.1.2190"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-095910"},{"issue":"8","key":"30","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.4049\/jimmunol.163.8.4175","volume":"163","year":"1999","journal-title":"Journal of Immunology"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/589494"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.140"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.4161\/viru.29092"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2001.01258.x"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00030"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1186\/s12865-015-0095-y"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166582"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-S6-S3"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/6535479.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/6535479.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/6535479.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T15:16:07Z","timestamp":1692976567000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/6535479\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":38,"alternative-id":["6535479","6535479"],"URL":"https:\/\/doi.org\/10.1155\/2017\/6535479","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2017]]}}}