{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:30:19Z","timestamp":1777613419439,"version":"3.51.4"},"reference-count":66,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T00:00:00Z","timestamp":1484092800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Recent study has demonstrated the gastroprotective activity of crude methanolic extract of <jats:italic>M. malabathricum<\/jats:italic> leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extract followed by the elucidation of the gastroprotective mechanisms of the most effective partition. Using the ethanol\u2010induced gastric ulcer assay, all partitions exerted significant gastroprotection, with EAMM being the most effective partition. EAMM significantly (i) reduced the volume and acidity (free and total) while increasing the pH of gastric juice and enhanced the gastric wall mucus secretion when assessed using the pylorus ligation assay, (ii) increased the enzymatic and nonenzymatic antioxidant activity of the stomach tissue, (iii) lost its gastroprotective activity following pretreatment with N\u2010omega\u2010nitro\u2010L\u2010arginine methyl ester (L\u2010NAME; NO blocker) or carbenoxolone (CBXN; NP\u2010SH blocker), (iv) exerted antioxidant activity against various in vitro oxidation assays, and (v) showed moderate in vitro anti\u2010inflammatory activity via the LOX\u2010modulated pathway. In conclusion, EAMM exerts a remarkable NO\/NP\u2010SH\u2010dependent gastroprotective effect that is attributed to its antisecretory and antioxidant activities, ability to stimulate the gastric mucus production and endogenous antioxidant system, and synergistic action of several gastroprotective\u2010induced flavonoids.<\/jats:p>","DOI":"10.1155\/2017\/6542631","type":"journal-article","created":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T21:03:17Z","timestamp":1484168597000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Semipurified Ethyl Acetate Partition of Methanolic Extract of <i>Melastoma malabathricum<\/i> Leaves Exerts Gastroprotective Activity Partly via Its Antioxidant\u2010Antisecretory\u2010Anti\u2010Inflammatory Action and Synergistic Action of Several Flavonoid\u2010Based Compounds"],"prefix":"10.1155","volume":"2017","author":[{"given":"Noor Wahida","family":"Ismail Suhaimy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad Khusairi","family":"Noor Azmi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norhafizah","family":"Mohtarrudin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4386-412X","authenticated-orcid":false,"given":"Maizatul Hasyima","family":"Omar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4772-1062","authenticated-orcid":false,"given":"Siti Farah Md.","family":"Tohid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manraj Singh","family":"Cheema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9117-9880","authenticated-orcid":false,"given":"Lay Kek","family":"Teh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9882-0661","authenticated-orcid":false,"given":"Mohd. 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