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Male rats were intragastrically fed for consecutive 8\u2010week and 4\u2010week recovery with the fruit of <jats:italic>J. Sabina<\/jats:italic>, and sperm maturation, serum testosterone level, and histopathology were analyzed. Epididymal epithelial cell culture was prepared for detection of podophyllotoxin activities. Furthermore, cell proliferation, transmission electron microscopy, Annexin V\/Propidium iodide, TUNEL, RT\u2010PCR, ELISA, and western blotting were examined. The results showed that rat sperm motility and fertility were remarkably declined after feeding the fruit. Moreover, the fruit targeted the epididymis rather than the testis. After 4\u2010week recovery, more than half of the male rats resumed normal fertility. It was found that podophyllotoxin significantly inhibited epididymal epithelial cell proliferation, promoted cell apoptosis, and increased the mRNA and protein levels of TNF\u2010<jats:italic>\u03b1<\/jats:italic> and the expression levels of cytochrome c, caspase\u20108, caspase\u20109, and caspase\u20103. Our findings suggest that the fruit of <jats:italic>J. sabina<\/jats:italic> could inhibit male rat sperm maturation and fertility. The potential mechanism might be related to podophyllotoxin, inducing epididymal epithelial cell apoptosis through TNF\u2010<jats:italic>\u03b1<\/jats:italic> and caspase signaling pathway.<\/jats:p>","DOI":"10.1155\/2017\/6958982","type":"journal-article","created":{"date-parts":[[2017,7,4]],"date-time":"2017-07-04T21:03:15Z","timestamp":1499202195000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Podophyllotoxin Extracted from <i>Juniperus sabina<\/i> Fruit Inhibits Rat Sperm Maturation and Fertility by Promoting Epididymal Epithelial Cell Apoptosis"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5753-6824","authenticated-orcid":false,"given":"Shuwu","family":"Xie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoting","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8682-5203","authenticated-orcid":false,"given":"Ruihua","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhigang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieyun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangjie","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aying","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5247-5978","authenticated-orcid":false,"given":"Yueying","family":"Qian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5136-7601","authenticated-orcid":false,"given":"Yan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,4]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0464"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.06.045"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2012.08.016"},{"key":"e_1_2_11_4_2","first-page":"S58","article-title":"Advances in male hormonal contraception","volume":"140","author":"Antonietta C.","year":"2014","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-1846"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(03)00135-1"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmi046"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(16)59418-7"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.1985.tb00844.x"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.12.039"},{"key":"e_1_2_11_11_2","unstructured":"QianY. 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