{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:28:26Z","timestamp":1783610906722,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY14H280006"],"award-info":[{"award-number":["LY14H280006"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["Y201121241"],"award-info":[{"award-number":["Y201121241"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Founds of Zhejiang Provincial Educational Department","award":["LY14H280006"],"award-info":[{"award-number":["LY14H280006"]}]},{"name":"Founds of Zhejiang Provincial Educational Department","award":["Y201121241"],"award-info":[{"award-number":["Y201121241"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gastroenterology Research and Practice"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>We used human gastric epithelial cells (GES-1) line in an ethanol-induced cell damage model to study the protective effect of<jats:italic>Veronicastrum axillare<\/jats:italic>and its modulation to NF-<jats:italic>\u03ba<\/jats:italic>B signal pathway. The goal was to probe the molecular mechanism of<jats:italic>V. axillare<\/jats:italic>decoction in the prevention of gastric ulcer and therefore provide guidance in the clinical application of<jats:italic>V. axillare<\/jats:italic>on treating injuries from chronic nephritis, pleural effusion, gastric ulcer, and other ailments. The effects of<jats:italic>V. axillare<\/jats:italic>-loaded serums on cell viability were detected by MTT assays. Enzyme-linked immunosorbent assay (ELISA) and Real-Time PCR methods were used to analyze the protein and mRNA expression of TNF-<jats:italic>\u03b1<\/jats:italic>, NF-<jats:italic>\u03ba<\/jats:italic>B, I<jats:italic>\u03ba<\/jats:italic>B<jats:italic>\u03b1<\/jats:italic>, and IKK<jats:italic>\u03b2<\/jats:italic>. The results showed that<jats:italic>V. axillare<\/jats:italic>-loaded serum partially reversed the damaging effects of ethanol and NF-<jats:italic>\u03ba<\/jats:italic>B activator (phorbol-12-myristate-13-acetate: PMA) and increased cell viability. The protein and mRNA expressions of TNF-<jats:italic>\u03b1<\/jats:italic>, NF-<jats:italic>\u03ba<\/jats:italic>B, I<jats:italic>\u03ba<\/jats:italic>B<jats:italic>\u03b1<\/jats:italic>, and IKK<jats:italic>\u03b2<\/jats:italic>were significantly upregulated by ethanol and PMA while they were downregulated by<jats:italic>V. axillare<\/jats:italic>-loaded serum. In summary,<jats:italic>V. axillare<\/jats:italic>-loaded serum has significantly protective effect on GES-1 against ethanol-induced injury. The protective effect was likely linked to downregulation of TNF-<jats:italic>\u03b1<\/jats:italic>based NF-<jats:italic>\u03ba<\/jats:italic>B signal pathway.<\/jats:p>","DOI":"10.1155\/2017\/7395032","type":"journal-article","created":{"date-parts":[[2017,1,15]],"date-time":"2017-01-15T16:02:55Z","timestamp":1484496175000},"page":"1-8","source":"Crossref","is-referenced-by-count":11,"title":["<i>Veronicastrum axillare<\/i>Alleviates Ethanol-Induced Injury on Gastric Epithelial Cells via Downregulation of the NF-kB Signaling Pathway"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7264-3978","authenticated-orcid":true,"given":"Wei-chun","family":"Zhao","sequence":"first","affiliation":[{"name":"College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-shan","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada V5A 1S6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan-yi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui-bin","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i39.5196"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.2.178"},{"key":"3","year":"2014"},{"issue":"10","key":"4","first-page":"1525","volume":"24","year":"2002","journal-title":"Guangxi Medical Journal"},{"issue":"1","key":"5","first-page":"122","volume":"13","year":"2011","journal-title":"Journal of Military Surgeon in Southwest China"},{"issue":"33","key":"6","first-page":"52","volume":"18","year":"2012","journal-title":"Contemporary Medicine"},{"issue":"4","key":"7","first-page":"1239","volume":"4","year":"2013","journal-title":"Journal of Food Safety and Food Quality"},{"key":"8","first-page":"19801","volume":"32","year":"2011","journal-title":"Journal of Anhui Agricultural Sciences"},{"key":"9","volume":"21, article 44","year":"2010","journal-title":"New Rural Technology"},{"issue":"11","key":"10","first-page":"516","year":"1997","journal-title":"Zhejiang Journal of Traditional Chinese Medicine"},{"key":"11","volume":"1, article 33","year":"2003","journal-title":"Zhejiang Journal of Traditional Chinese Medicine"},{"issue":"2","key":"12","first-page":"148","volume":"19","year":"2005","journal-title":"Journal of Practical Traditional Chinese Internal Medicine"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5168"},{"issue":"10","key":"14","first-page":"1370","volume":"32","year":"2012","journal-title":"Chinese Journal of Integrated Traditional and Western Medicine"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/s2221-1691(13)60180-x"},{"issue":"10","key":"16","first-page":"1151","volume":"37","year":"2013","journal-title":"Journal of Chinese Medical University"},{"key":"17","year":"2014"},{"issue":"1","key":"18","first-page":"13","volume":"40","year":"2016","journal-title":"Journal of Zheijang Chinese Medical University"},{"issue":"3","key":"19","first-page":"173","volume":"40","year":"2016","journal-title":"Journal of Zhejiang Chinese Medical University"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.2144\/000112401"},{"issue":"3","key":"21","first-page":"532","volume":"15","year":"1993","journal-title":"BioTechniques"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9440(10)61708-7"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.16.1.225"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.14.1.649"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2010.03390.x"},{"key":"26","year":"2012"},{"issue":"11","key":"27","first-page":"1005","volume":"34","year":"2014","journal-title":"Recent Advances in Ophthalmology"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80042-4"},{"key":"29","year":"2007"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m109358200"},{"issue":"5","key":"31","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1360\/csb2002-47-5-323","volume":"47","year":"2002","journal-title":"Chinese Science Bulletin"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m110572200"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(01)00049-7"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.1.28"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/s0161-5890(99)00079-6"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-200401000-00026"},{"issue":"7","key":"37","first-page":"15","volume":"23","year":"2012","journal-title":"Hainan Medical Journal"},{"issue":"6","key":"38","first-page":"480","volume":"19","year":"2009","journal-title":"Journal of Jiangsu University (Medicine Edition)"}],"container-title":["Gastroenterology Research and Practice"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/grp\/2017\/7395032.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/grp\/2017\/7395032.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/grp\/2017\/7395032.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,9,17]],"date-time":"2019-09-17T11:36:58Z","timestamp":1568720218000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/grp\/2017\/7395032\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":38,"alternative-id":["7395032","7395032"],"URL":"https:\/\/doi.org\/10.1155\/2017\/7395032","relation":{},"ISSN":["1687-6121","1687-630X"],"issn-type":[{"value":"1687-6121","type":"print"},{"value":"1687-630X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}