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The response of the biosensor as a function of the relative concentration of the two immobilized enzymes was investigated and the best working conditions were identified by measuring the sensitivity and the linear range response. In particular, our best lactose biosensor shows a linear range up to 0.010\u2009mM and a limit of detection (LOD) and a sensitivity equal to 0.001\u2009mM and 850 \u00b1 81\u2009<jats:italic>\u03bc<\/jats:italic>A\/mM, respectively. For glucose, the values of the above-mentioned parameters are equal to 0.015\u2009mM for the linear range, 0.001\u2009mM for LOD, and 505 \u00b1 55\u2009<jats:italic>\u03bc<\/jats:italic>A\/mM for the sensitivity. The working parameters of our biosensors are significant in comparison with other biosensors developed for concentration determination of the two saccharides investigated in the present work. In particular, low (LOD) and high sensitivities are obtained for lactose and glucose. To challenge our biosensor with real samples, it was tested using fruit juices, skim milk, and whey.<\/jats:p>","DOI":"10.1155\/2017\/7498945","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T17:02:11Z","timestamp":1502989331000},"page":"1-8","source":"Crossref","is-referenced-by-count":15,"title":["Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor"],"prefix":"10.1155","volume":"2017","author":[{"given":"Marianna","family":"Portaccio","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universit\u00e0 della Campania \u201cLuigi Vanvitelli\u201d, Via S.M. di Costantinopoli, 16-80134 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-5904","authenticated-orcid":true,"given":"Maria","family":"Lepore","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Universit\u00e0 della 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