{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:06:20Z","timestamp":1791846380430,"version":"4.3.4"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T00:00:00Z","timestamp":1511395200000},"content-version":"vor","delay-in-days":326,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Five medicinal plants of Pakistan were investigated for their antinociceptive, anti\u2010inflammatory, antidepressant, and anticoagulant potential. Antinociceptive activity was estimated by hot plate and writhing assay. In hot plate assay,<jats:italic>Quercus dilatata<\/jats:italic>(52.2%) and<jats:italic>Hedera nepalensis<\/jats:italic>(59.1%) showed moderate while<jats:italic>Withania coagulans<\/jats:italic>(65.3%) displayed a significant reduction in pain. On the other hand, in writhing assay,<jats:italic>Quercus dilatata<\/jats:italic>(49.6%),<jats:italic>Hedera nepalensis<\/jats:italic>(52.7%), and<jats:italic>Withania coagulans<\/jats:italic>(62.0%) showed comparative less activity. In anti\u2010inflammatory assays crude extracts showed significant edema inhibition in a dose dependent manner. In carrageenan assay, the highest activity was observed for<jats:italic>Withania coagulans<\/jats:italic>(70.0%) followed by<jats:italic>Quercus dilatata<\/jats:italic>(66.7%) and<jats:italic>Hedera nepalensis<\/jats:italic>(63.3%). Similar behavior was observed in histamine assay with percentage inhibitions of 74.3%, 60.4%, and 63.5%, respectively. Antidepressant activity was estimated by forced swim test and the most potent activity was revealed by<jats:italic>Withania coagulans<\/jats:italic>with immobility time 2.2s (95.9%) followed by<jats:italic>Hedera nepalensis<\/jats:italic>with immobility time 25.3s (53.4%). Moreover, the crude extracts of<jats:italic>Fagonia cretica<\/jats:italic>(74.6%),<jats:italic>Hedera nepalensis<\/jats:italic>(73.8%), and<jats:italic>Phytolacca latbenia<\/jats:italic>(67.3%) showed good anticoagulant activity with coagulation times 86.9s, 84.3s, and 67.5s, respectively. Collectively, the results demonstrate that these five plants have rich medicinal constituents which can be further explored.<\/jats:p>","DOI":"10.1155\/2017\/7849501","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T23:31:39Z","timestamp":1511479899000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Five Indigenous Plants of Pakistan with Antinociceptive, Anti\u2010Inflammatory, Antidepressant, and Anticoagulant Properties in Sprague Dawley Rats"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8008-4595","authenticated-orcid":false,"given":"Hammad","family":"Ismail","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammara","family":"Rasheed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ihsan-ul","family":"Haq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laila","family":"Jafri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazif","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erum","family":"Dilshad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moniba","family":"Sajid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4134-6636","authenticated-orcid":false,"given":"Bushra","family":"Mirza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,23]]},"reference":[{"key":"e_1_2_8_1_2","article-title":"Medicinal plants of Pakistan: Challenges and Opportunities","volume":"6","author":"Ullah N.","year":"2017","journal-title":"Journal of Complementary & Alternative Medici"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/np9604893"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/627375"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nel034"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/302426"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1245-3"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-016-0811-7"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.3923\/ajps.2004.410.415"},{"key":"e_1_2_8_9_2","first-page":"359","article-title":"Pollen morphology of three species of quercus (Family Fagaceae) [Quercus dilatata, Q. incana and Q. ballota]","volume":"1","author":"Shah S.","year":"2005","journal-title":"Journal of Agriculture and Social Sciences"},{"key":"e_1_2_8_10_2","first-page":"39","article-title":"Traditional uses of medicinal plants of Nandiar Khuwarr catchment (District Battagram), Pakistan","volume":"5","author":"Haq F.","year":"2011","journal-title":"Journal of Medicinal Plants Research"},{"key":"e_1_2_8_11_2","first-page":"577","article-title":"HPLC-DAD analysis and free radical scavenging potential of Quercus dilatata L","volume":"45","author":"Ihtisham M.","year":"2013","journal-title":"Pakistan Journal of Botany"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.4314\/tjpr.v7i1.14677"},{"key":"e_1_2_8_13_2","first-page":"19","article-title":"Cytotoxic and antitumor potential of Fagonia cretica L","volume":"31","author":"Hussain A.","year":"2007","journal-title":"Turkish Journal of Biology"},{"key":"e_1_2_8_14_2","volume-title":"Medicinal and poisonous plants of Pakistan","author":"Baquar S. 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