{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T19:13:32Z","timestamp":1780773212720,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,2,12]],"date-time":"2017-02-12T00:00:00Z","timestamp":1486857600000},"content-version":"vor","delay-in-days":42,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>This study aimed to evaluate the leishmanicidal activity of ethanol extract, fractions, and isolated substance from <jats:italic>Handroanthus serratifolius<\/jats:italic> against <jats:italic>Leishmania amazonensis<\/jats:italic>. Furthermore, this activity was related to cytotoxicity, and the selectivity index was determined. The ethanol extract was obtained by maceration of the stem powder, and the extract was subjected to fractionation on chromatographic column. The lapachol was obtained by acid base extraction followed by purification in chromatographic column. The antipromastigote activity and cytotoxicity tests were carried out by the cell viability method (MTT). Modified THP\u20101 cells were infected with <jats:italic>L. amazonensis<\/jats:italic> promastigotes and treated for 24\u2009h with different concentrations of the extract, fractions, and lapachol. The ethanol extract, dichloromethane, and ethyl acetate fractions were not active against promastigotes (IC<jats:sub>50<\/jats:sub> &gt; 200\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) or cytotoxic (CC<jats:sub>50<\/jats:sub> &gt; 500\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL), and the selectivity index (SI) was greater than 2.5. The ethyl acetate fraction was active only in promastigotes; it is not cytotoxic (CC<jats:sub>50<\/jats:sub> &gt; 500\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL, SI &gt; 5). The lapachol was selectively active only against amastigote (IS &gt; 2.5, CC<jats:sub>50<\/jats:sub> &gt; 500\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL). In summary, lapachol and ethyl acetate fraction are promising against amastigote and promastigote forms, respectively.<\/jats:p>","DOI":"10.1155\/2017\/8074275","type":"journal-article","created":{"date-parts":[[2017,2,12]],"date-time":"2017-02-12T21:00:45Z","timestamp":1486933245000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Antileishmanial Activity of <i>Handroanthus serratifolius<\/i> (Vahl) S. 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