{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T10:21:23Z","timestamp":1768731683216,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372091"],"award-info":[{"award-number":["81372091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471880"],"award-info":[{"award-number":["81471880"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301647"],"award-info":[{"award-number":["81301647"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372091"],"award-info":[{"award-number":["81372091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471880"],"award-info":[{"award-number":["81471880"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301647"],"award-info":[{"award-number":["81301647"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81372091"],"award-info":[{"award-number":["81372091"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471880"],"award-info":[{"award-number":["81471880"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81301647"],"award-info":[{"award-number":["81301647"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Expedited bone tissue engineering employs the biological stimuli to harness the intrinsic regenerative potential of skeletal muscle to trigger the reparative process in situ to improve or replace biological functions. When genetically modified with adenovirus mediated BMP2 gene transfer, muscle biopsies from animals have demonstrated success in regenerating bone within rat bony defects. However, it is uncertain whether the human adult skeletal muscle displays an osteogenic potential in vitro when a suitable biological trigger is applied. In present study, human skeletal muscle cultured in a standard osteogenic medium supplemented with dexamethasone demonstrated significant increase in alkaline phosphatase activity approximately 24-fold over control at 2-week time point. More interestingly, measurement of mRNA levels revealed the dramatic results for osteoblast transcripts of alkaline phosphatase, bone sialoproteins, transcription factor CBFA1, collagen type I, and osteocalcin. Calcified mineral deposits were demonstrated on superficial layers of muscle discs after an extended 8-week osteogenic induction. Taken together, these are the first data supporting human skeletal muscle tissue as a promising potential target for expedited bone regeneration, which of the technologies is a valuable method for tissue repair, being not only effective but also inexpensive and clinically expeditious.<\/jats:p>","DOI":"10.1155\/2017\/8619385","type":"journal-article","created":{"date-parts":[[2017,1,22]],"date-time":"2017-01-22T21:02:09Z","timestamp":1485118929000},"page":"1-7","source":"Crossref","is-referenced-by-count":5,"title":["Osteogenic Differentiation Capacity of In Vitro Cultured Human Skeletal Muscle for Expedited Bone Tissue Engineering"],"prefix":"10.1155","volume":"2017","author":[{"given":"Chunlei","family":"Miao","sequence":"first","affiliation":[{"name":"Institute of Plastic and Reconstructive Surgery, Hospital for Plastic Surgery Affiliated to Weifang Medical University, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lulu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Plastic and Reconstructive Surgery, Hospital for Plastic Surgery Affiliated to Weifang Medical University, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lufeng","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Orthopedic and Trauma Surgery, Weifang People\u2019s Hospital, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Eugenom Inc., San Diego, CA 92121, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Plastic and Reconstructive Surgery, Hospital for Plastic Surgery Affiliated to Weifang Medical University, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanghong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Weifang People\u2019s Hospital, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plastic and Cosmetic Surgery, Huadong Hospital Affiliated to Fudan University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1523-9797","authenticated-orcid":true,"given":"Shengjian","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Plastic and Reconstructive Surgery, Hospital for Plastic Surgery Affiliated to Weifang Medical University, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2769-5012","authenticated-orcid":true,"given":"Fangjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Plastic and Reconstructive Surgery, Hospital for Plastic Surgery Affiliated to Weifang Medical University, Weifang, Shandong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000194677.02506.45"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2002.0801"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3944\/aott.2012.2671"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2006.02.040"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.03.016"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1038\/nm1295-1322"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1002\/jor.22038"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0302"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2008.05.013"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100180613"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.22203\/ecm.v019a10"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.471268"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/6529"},{"issue":"367","key":"14","first-page":"S68","year":"1999","journal-title":"Clinical Orthopaedics and Related Research"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.11.038"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0052"},{"issue":"1","key":"17","doi-asserted-by":"crossref","first-page":"139","DOI":"10.2174\/1874210601105010139","volume":"5","year":"2011","journal-title":"Open Dentistry Journal"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2010.01224.x"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2010.12.006"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.07.005"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1615\/critrevbiomedeng.v40.i5.10"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1097\/00006534-200102000-00027"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1179\/016164104225013842"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-002-1004-5"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.5.1085"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v030a09"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056641"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.04.113"},{"key":"29","first-page":"85","volume":"1","year":"1991","journal-title":"Critical Reviews in Eukaryotic Gene Expression"},{"issue":"8","key":"30","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1093\/clinchem\/45.8.1359","volume":"45","year":"1999","journal-title":"Clinical Chemistry"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2005.07.034"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/s0020-1383(13)70008-1"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-011-1301-z"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/8619385.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/8619385.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/8619385.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T10:48:26Z","timestamp":1601376506000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/8619385\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":33,"alternative-id":["8619385","8619385"],"URL":"https:\/\/doi.org\/10.1155\/2017\/8619385","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}