{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:25:20Z","timestamp":1740144320632,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006020","name":"CTMM","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006020","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Weijerhorst Foundation"},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Contrast Media &amp; Molecular Imaging"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>As CD13 is selectively expressed in angiogenesis, it can serve as a target for molecular imaging tracers to noninvasively visualize angiogenic processes in vivo. The CD13-targeting moiety NGR was synthesized and cyclized by native chemical ligation (NCL) instead of disulfide bridging, leading to a cyclic peptide backbone: cyclo(Cys-Asn-Gly-Arg-Gly) (coNGR). Beside this new monomeric coNGR, a tetrameric NGR peptide co(NGR)<jats:sub>4<\/jats:sub> was designed and synthesized. After radiolabeling, their in vitro and in vivo characteristics were determined. Both coNGR-based imaging agents displayed considerably higher standardized uptake values (SUVs) at infarcted areas compared to the previously reported disulfide-cyclized cNGR imaging agent. Uptake patterns of <jats:sup>111<\/jats:sup>In-coNGR and <jats:sup>111<\/jats:sup>In-co(NGR)<jats:sub>4<\/jats:sub> coincided with CD13 immunohistochemistry on excised hearts. Blood stability tests indicated better stability for both novel imaging agents after 50\u2009min blood incubation compared to the disulfide-cyclized cNGR imaging agent. In mice, both coNGR peptides cleared rapidly from the blood mainly via the kidneys. In addition, co(NGR)<jats:sub>4<\/jats:sub> showed a significantly higher specific uptake in infarcted myocardium compared to coNGR and thus is a promising sensitive imaging agent for detection of angiogenesis in infarcted myocardium.<\/jats:p>","DOI":"10.1155\/2017\/8638549","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T19:33:10Z","timestamp":1508873590000},"page":"1-9","source":"Crossref","is-referenced-by-count":3,"title":["Use of Cyclic Backbone NGR-Based SPECT to Increase Efficacy of Postmyocardial Infarction Angiogenesis Imaging"],"prefix":"10.1155","volume":"2017","author":[{"given":"Geert","family":"Hendrikx","sequence":"first","affiliation":[{"name":"Department of Radiology and Nuclear Medicine, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University Medical Center (MUMC+), Maastricht, Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tilman 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