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Many studies indicated that antioxidants protect against radiation\u2010induced damage and have high efficacy and lack of toxicity in preventing radiation exposure damages. The purpose of this study was to investigate the <jats:italic>in vitro<\/jats:italic> protective effect of a new antioxidant mixture, named RiduROS, on oxidative stress generation and DNA double\u2010strand breaks (DSBs) induced by low doses of X\u2010rays in endothelial cells. Human umbilical vein endothelial cells (HUVEC) were treated with RiduROS mixture 24\u2009h before a single exposure to X\u2010rays at an absorbed dose of 0.25\u2009Gy. The production of reactive oxygen species (ROS) was evaluated by fluorescent dye staining and nitric oxide (NO) by the Griess reaction, and DSBs were evaluated as number of <jats:italic>\u03b3<\/jats:italic>\u2010H2AX foci. We demonstrated that antioxidant mixture reduced oxidative stress induced by low dose of X\u2010ray irradiation and that RiduROS pretreatment is more effective in protecting against radiation\u2010induced oxidative stress than single antioxidants. Moreover, RiduROS mixture is able to reduce <jats:italic>\u03b3<\/jats:italic>\u2010H2AX foci formation after low\u2010dose X\u2010ray exposure. The texted mixture of antioxidants significantly reduced oxidative stress and <jats:italic>\u03b3<\/jats:italic>\u2010H2AX foci formation in endothelial cells exposed to low\u2010dose irradiation. These results suggest that RiduROS could have a role as an effective radioprotectant against low\u2010dose damaging effects.<\/jats:p>","DOI":"10.1155\/2017\/9085947","type":"journal-article","created":{"date-parts":[[2017,8,9]],"date-time":"2017-08-09T21:06:41Z","timestamp":1502312801000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A New Natural Antioxidant Mixture Protects against Oxidative and DNA Damage in Endothelial Cell Exposed to Low\u2010Dose 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