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This paper describes in vitro and in vivo preclinical studies of two new reassortants of pandemic H1N1 live attenuated influenza vaccine (LAIV) candidates. One had the hemagglutinin (HA) and neuraminidase (NA) genes from A\/South Africa\/3626\/2013 H1N1 wild-type virus on the A\/Leningrad\/134\/17\/57 master donor virus backbone (6\u2009:\u20092 formulation) while the second had the HA, NA, and NP genes of the wild-type virus on the same backbone (5\u2009:\u20093 formulation). Although both LAIVs induced similar antibody immune responses, the 5\u2009:\u20093 LAIV provoked greater production of virus-specific CTLs than the 6\u2009:\u20092 variant. Furthermore, the 5\u2009:\u20093 LAIV-induced CTLs had higher in vivo cytotoxic activity, compared to 6\u2009:\u20092 LAIV. Finally, the 5\u2009:\u20093 LAIV candidate afforded greater protection against infection and severe illness than the 6\u2009:\u20092 LAIV. Inclusion in LAIV of the NP gene from wild-type influenza virus is a new approach to inducing cross-reactive cell-mediated immune responses and cross protection against pandemic influenza.<\/jats:p>","DOI":"10.1155\/2017\/9359276","type":"journal-article","created":{"date-parts":[[2017,1,22]],"date-time":"2017-01-22T21:02:40Z","timestamp":1485118960000},"page":"1-11","source":"Crossref","is-referenced-by-count":22,"title":["Immunogenicity and Cross Protection in Mice Afforded by Pandemic H1N1 Live Attenuated Influenza Vaccine Containing Wild-Type Nucleoprotein"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2156-1635","authenticated-orcid":true,"given":"Andrey","family":"Rekstin","sequence":"first","affiliation":[{"name":"Institute of Experimental Medicine, Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Isakova-Sivak","sequence":"additional","affiliation":[{"name":"Institute of 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