{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T13:54:37Z","timestamp":1762955677074,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011774","name":"Laboratorio de Inmunolog\u00eda y Virolog\u00eda de la Universidad Aut\u00f3noma de Nuevo Le\u00f3n","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011774","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2017]]},"abstract":"<jats:p>Nephrotic syndrome (NS) is a glomerular disease that is defined by the leakage of protein into the urine and is associated with hypoalbuminemia, hyperlipidemia, and edema. Steroid-resistant NS (SRNS) patients do not respond to treatment with corticosteroids and show decreased Wilms tumor 1 (WT1) expression in podocytes. Downregulation of WT1 has been shown to be affected by certain microRNAs (miRNAs). Twenty-one patients with idiopathic NS (68.75% were SSNS and 31.25% SRNS) and 10 healthy controls were enrolled in the study. Podocyte number and WT1 location were determined by immunofluorescence, and the serum levels of miR-15a, miR-16-1, and miR-193a were quantified by RT-qPCR. Low expression and delocalization of WT1 protein from the nucleus to the cytoplasm were found in kidney biopsies of patients with SRNS and both nuclear and cytoplasmic localization were found in steroid-sensitive NS (SSNS) patients. In sera from NS patients, low expression levels of miR-15a and miR-16-1 were found compared with healthy controls, but only the miR-16-1 expression levels showed statistically significant decrease (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.019<\/mml:mn><\/mml:math>). The miR-193a expression levels only slightly increased in NS patients. We concluded that low expression and delocalization from the WT1 protein in NS patients contribute to loss of podocytes while modulation from WT1 protein is not associated with the miRNAs analyzed in sera from the patients.<\/jats:p>","DOI":"10.1155\/2017\/9531074","type":"journal-article","created":{"date-parts":[[2017,2,19]],"date-time":"2017-02-19T21:02:40Z","timestamp":1487538160000},"page":"1-8","source":"Crossref","is-referenced-by-count":11,"title":["Cytoplasmic Localization of WT1 and Decrease of miRNA-16-1 in Nephrotic Syndrome"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2986-0755","authenticated-orcid":true,"given":"Pablo","family":"Zapata-Benavides","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), 66450 San Nicol\u00e1s de los Garza, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-6471","authenticated-orcid":true,"given":"Mariela","family":"Arellano-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), 66450 San Nicol\u00e1s de los Garza, NL, Mexico"},{"name":"Laboratorios de Inmunolog\u00eda y Biolog\u00eda Molecular, Unidad Acad\u00e9mica de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Zacatecas (UAZ), 98040 Zacatecas, ZAC, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9767-4834","authenticated-orcid":true,"given":"Juan Jos\u00e9","family":"Bollain-y-Goytia","sequence":"additional","affiliation":[{"name":"Laboratorios de Inmunolog\u00eda y Biolog\u00eda Molecular, Unidad Acad\u00e9mica de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Zacatecas (UAZ), 98040 Zacatecas, ZAC, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0522-6637","authenticated-orcid":true,"given":"Mois\u00e9s Armides","family":"Franco-Molina","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), 66450 San Nicol\u00e1s de los Garza, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria Azucena","family":"Rangel-Ochoa","sequence":"additional","affiliation":[{"name":"Hospital General Zacatecas \u201cLuz Gonz\u00e1lez Cos\u00edo\u201d, Ciudad Gobierno, 98160 Zacatecas, ZAC, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3403-9849","authenticated-orcid":true,"given":"Esperanza","family":"Avalos-D\u00edaz","sequence":"additional","affiliation":[{"name":"Laboratorios de Inmunolog\u00eda y Biolog\u00eda Molecular, Unidad Acad\u00e9mica de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Zacatecas (UAZ), 98040 Zacatecas, ZAC, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6516-1188","authenticated-orcid":true,"given":"Rafael","family":"Herrera-Esparza","sequence":"additional","affiliation":[{"name":"Laboratorios de Inmunolog\u00eda y Biolog\u00eda Molecular, Unidad Acad\u00e9mica de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Zacatecas (UAZ), 98040 Zacatecas, ZAC, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Rodr\u00edguez-Padilla","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda e Inmunolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad Aut\u00f3noma de Nuevo Le\u00f3n (UANL), 66450 San Nicol\u00e1s de los Garza, NL, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1681\/asn.2014030267"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2014.00226"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2013.247"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-012-0776-y"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/782395"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00056.2013"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006040325"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.4238\/2013.December.4.5"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.4238\/2014.November.11.16"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1093\/ndtplus\/sfq173"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1203\/01.pdr.0000196717.94518.f0"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-30-110"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140713078"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00050"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2015.150407"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12585"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/731479"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12420"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-31-27"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3142"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/593956"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.31.363"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00462.2010"},{"issue":"5","key":"24","first-page":"371","volume":"8","year":"2014","journal-title":"Iranian Journal of Kidney Diseases"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010344"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"issue":"4","key":"27","first-page":"CD001533","year":"2007","journal-title":"Cochrane database of systematic reviews (Online)"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1186\/s13052-015-0123-9"},{"key":"29","first-page":"557","volume-title":"Steroid-resistant idiopathic nephrotic syndrome in children","volume":"2004","year":"2004"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.4103\/0971-4065.83029"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12227"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073798"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00056.2013"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1111\/apa.12317"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00775.x"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.6.651"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04622.x"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2009.69"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/9531074.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/9531074.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2017\/9531074.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T16:05:24Z","timestamp":1589731524000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2017\/9531074\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":38,"alternative-id":["9531074","9531074"],"URL":"https:\/\/doi.org\/10.1155\/2017\/9531074","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2017]]}}}