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BF was fractionated by reversed\u2010phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to HSCCC, where patuletin and patulitrin were isolated. CAE and the fractions BF, EAF, DF, and AQF were analyzed by LC\u2010DAD\u2010MS, and patuletin and patulitrin were determined as the major substances in EAF and BF, respectively. BF was also analyzed by HPLC and capillary electrophoresis (CE), and patulitrin was again determined to be the main substance in this fraction. CAE and the semipurified fractions (750, 500, 300, 100, and 50\u2009mg\/L) were assayed for larvicidal activity against <jats:italic>Aedes aegypti<\/jats:italic>, with mortality rate expressed as percentage. All fractions except AQF showed insecticidal activity after 24\u2009h exposure of larvae to the highest concentration. However, EAF showed the highest activity with more than 50% reduction in larval population at 50\u2009mg\/L. The insecticidal activity observed with EAF might have been due to the higher concentration of patuletin present in this fraction.<\/jats:p>","DOI":"10.1155\/2017\/9602368","type":"journal-article","created":{"date-parts":[[2017,12,7]],"date-time":"2017-12-07T23:31:17Z","timestamp":1512689477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Larvicidal Activity against <i>Aedes aegypti<\/i> and Chemical Characterization of the Inflorescences of <i>Tagetes patula<\/i>"],"prefix":"10.1155","volume":"2017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1405-8751","authenticated-orcid":false,"given":"Let\u00edcia Maria","family":"Krzyzaniak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia Mara","family":"Antonelli-Ushirobira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gean","family":"Panizzon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Luiza","family":"Sereia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Roberto Pinto de","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Antonio Cyrino","family":"Zequi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio Roberto","family":"Novello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisely Cristiny","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela Cristina de","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise Brentan","family":"Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eneri Vieira de Souza","family":"Leite-Mello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7532-3395","authenticated-orcid":false,"given":"Jo\u00e3o Carlos Palazzo de","family":"Mello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,7]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0034-89102008000400011"},{"volume-title":"Dengue: Guidelines for Diagnosis, Treatment, Preventions and Control","year":"2009","author":"World Health Organization","key":"e_1_2_7_2_2"},{"key":"e_1_2_7_3_2","unstructured":"World Health Organization Dengue and severe dengue 2017."},{"key":"e_1_2_7_4_2","unstructured":"Whopes World Health Organization Pesticides Evaluation Scheme 2016."},{"volume-title":"Parasitologia humana","year":"2011","author":"Eiras A. 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