{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:57:58Z","timestamp":1753887478870,"version":"3.41.2"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,12]],"date-time":"2017-11-12T00:00:00Z","timestamp":1510444800000},"content-version":"vor","delay-in-days":315,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771274","81500253"],"award-info":[{"award-number":["31771274","81500253"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p><jats:italic>Artemisia annua<\/jats:italic>L. belongs to the Asteraceae family, which is indigenous to China. It has valuable pharmacological properties, such as antimalarial, anti\u2010inflammatory, and anticancer properties. However, whether it possesses antiasthma properties is unknown. In the current study, chloroform extract of<jats:italic>Artemisia annua<\/jats:italic>L. (CEAA) was prepared, and we found that CEAA completely eliminated acetylcholine (ACh) or high K<jats:sup>+<\/jats:sup>\u2010elicited (80\u2009mM) contractions of mouse tracheal rings (TRs). Patch\u2010clamp technique and ion channel blockers were employed to explore the underlying mechanisms of the relaxant effect of CEAA. In whole\u2010cell current recording, CEAA almost fully abolished voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup>channel (VDCC) currents and markedly enhanced large conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup>(BK) channel currents on airway smooth muscle cells (ASMCs). In single channel current recording, CEAA increased the opening probability but had no effect on the single channel conductance of BK channels. However, under paxilline\u2010preincubated (a selective BK channel blocker) conditions, CEAA only slightly increased BK channel currents. These results indicate that CEAA may contain active components with potent antiasthma activity. The abolished VDCCs by CEAA may mainly contribute to the underlying mechanism through which it acts as an effective antiasthmatic compound, but the enhanced BK currents might play a less important role in the antiasthmatic effects.<\/jats:p>","DOI":"10.1155\/2017\/9870414","type":"journal-article","created":{"date-parts":[[2017,11,12]],"date-time":"2017-11-12T23:33:28Z","timestamp":1510529608000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Chloroform Extract of<i>Artemisia annua<\/i>L. Relaxes Mouse Airway Smooth Muscle"],"prefix":"10.1155","volume":"2017","author":[{"given":"Jun","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Qun","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongle","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nana","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congli","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3914-0775","authenticated-orcid":false,"given":"Qing-hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6795-4501","authenticated-orcid":false,"given":"Jinhua","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,12]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.130.1_suppl.4s"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61362-3"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0804579"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03256657"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200565120-00001"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00159708"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00085606"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-123"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.06.052"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.11"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-4902-z"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2017.02.009"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2015.11.001"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5626"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/591195"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-016-2077-9"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101578"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1540\/jsmr1965.20.427"},{"key":"e_1_2_10_19_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0031-6997(24)01321-8","article-title":"Calcium movements, distribution, and functions in smooth muscle","volume":"49","author":"Karaki H.","year":"1997","journal-title":"Pharmacological Reviews"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1442-2042.2002.00548.x"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.1995.268.2.L221"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02110699"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(92)90017-T"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/372231a0"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.85"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.278.2.C235"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800447200"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.204347"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1139\/Y07-060"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.58.030196.000555"},{"key":"e_1_2_10_31_2","first-page":"429","article-title":"Potassium channel modulation: A new drug principle for regulation of smooth muscle contractility. Studies on isolated airways and arteries","volume":"43","author":"Nielsen-Kudsk J. E.","journal-title":"Danish Medical Journal"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.1.67"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2017\/9870414.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2017\/9870414.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2017\/9870414","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:10:56Z","timestamp":1750983056000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2017\/9870414"}},"subtitle":[],"editor":[{"given":"Onesmo B.","family":"Balemba","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":32,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1155\/2017\/9870414"],"URL":"https:\/\/doi.org\/10.1155\/2017\/9870414","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"type":"print","value":"1741-427X"},{"type":"electronic","value":"1741-4288"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"2017-07-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9870414"}}