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In an attempt to better understand the pathogen-host interaction between the invading<jats:italic>M. ovipneumoniae<\/jats:italic>and airway epithelial cells, we investigated the host inflammatory responses against capsular polysaccharide (designated as CPS) of<jats:italic>M. ovipneumoniae<\/jats:italic>using sheep bronchial epithelial cells cultured in an air-liquid interface (ALI) model. Results showed that CPS derived from<jats:italic>M. ovipneumoniae<\/jats:italic>could activate toll-like receptor- (TLR-) mediated inflammatory responses, along with an elevated expression of nuclear factor kappa B (NF-<jats:italic>\u03ba<\/jats:italic>B), activator protein-1 (AP-1), and interferon regulatory factor 3 (IRF3) as well as various inflammatory-associated mediators, representatively including proinflammatory cytokines, such as IL1<jats:italic>\u03b2<\/jats:italic>, TNF<jats:italic>\u03b1<\/jats:italic>, and IL8, and anti-inflammatory cytokines such as IL10 and TGF<jats:italic>\u03b2<\/jats:italic>of TLR signaling cascade. Mechanistically, the CPS-induced inflammation was TLR initiated and was mediated by activations of both MyD88-dependent and MyD88-independent signaling pathways. Of importance, a blockage of CPS with specific antibody led a significant reduction of<jats:italic>M. ovipneumoniae<\/jats:italic>-induced inflammatory responses in sheep bronchial epithelial cells. These results suggested that CPS is a key virulent component of<jats:italic>M. ovipneumoniae<\/jats:italic>, which may play a crucial role in the inflammatory response induced by<jats:italic>M. ovipneumoniae<\/jats:italic>infections.<\/jats:p>","DOI":"10.1155\/2017\/9891673","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T21:01:21Z","timestamp":1493845281000},"page":"1-15","source":"Crossref","is-referenced-by-count":24,"title":["Capsular Polysaccharide is a Main Component of<i>Mycoplasma ovipneumoniae<\/i>in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells"],"prefix":"10.1155","volume":"2017","author":[{"given":"Zhongjia","family":"Jiang","sequence":"first","affiliation":[{"name":"Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Ningxia University, Ningxia 750021, China"},{"name":"College of Life Science, Ningxia 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