{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T11:14:44Z","timestamp":1784805284879,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T00:00:00Z","timestamp":1530662400000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003625","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["HI14C0955"],"award-info":[{"award-number":["HI14C0955"]}],"id":[{"id":"10.13039\/501100003625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Hepatitis B virus (HBV) infectious diseases currently remain incurable due to limitations of conventional antivirals such as incapability of eradicating HBV DNA, prolonged use, drug resistance, and virological relapse. KCT\u201001, a 30% ethanol extract consisting of <jats:italic>Artemisia capillaris<\/jats:italic>, <jats:italic>Sanguisorba officinalis<\/jats:italic>, and <jats:italic>Curcuma longa<\/jats:italic>, was newly developed. The objective of this study was to investigate pharmacological activities of KCT\u201001 against HBV using HepG2.2.15 cells and a hydrodynamic injection model. KCT\u201001 significantly lowered antigen secretion, virion production, and pgRNA synthesis in HepG2.2.15 cells without affecting cell viability. KCT\u201001 administration also resulted in significant decrease of serum virion production, liver covalently closed circular (ccc) DNA levels, and mRNA synthesis of cytokines in the liver of mice injected with HBV DNA hydrodynamically. Interestingly, coadministration of KCT\u201001 with entecavir enhanced its <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> antiviral activities. Moreover, safety of KCT\u201001 was assured up to 5000 mg\/kg in rats in both single and repeated\u2010dose preclinical studies. Taken together, our findings demonstrate that KCT\u201001 is capable of suppressing HBV replication and inflammatory cytokine production in <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> models without showing toxicity, suggesting the potential of using KCT\u201001 alone or in combination with entecavir as antiviral agent.<\/jats:p>","DOI":"10.1155\/2018\/1073509","type":"journal-article","created":{"date-parts":[[2018,7,4]],"date-time":"2018-07-04T23:30:43Z","timestamp":1530747043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Preclinical Evaluation of <i>In Vitro<\/i> and <i>In Vivo<\/i> Antiviral Activities of KCT\u201001, a New Herbal Formula against Hepatitis B Virus"],"prefix":"10.1155","volume":"2018","author":[{"given":"Hong","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eungyeong","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So-Young","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Yoon","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na-Rae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae-Sung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3141-3727","authenticated-orcid":false,"given":"Kyung-Tae","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3150-5338","authenticated-orcid":false,"given":"Kyung-Soo","family":"Inn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0085-6709","authenticated-orcid":false,"given":"Bum-Joon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4881-7357","authenticated-orcid":false,"given":"Jang-Hoon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,4]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(05)00384-7"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/epirev\/mxj009"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/nejm200205303462202"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70264-5"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22900"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22885"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23384"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.92.10.1619"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/728137"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.832"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1573(199705)11:3<179::AID-PTR50>3.3.CO;2-6"},{"key":"e_1_2_10_12_2","first-page":"447","article-title":"Inhibitory effects of Terminalia chebula, Sanguisorba officinalis, Rubus coreanus and Rheum palmatum on hepatitis B virus replication in HepG2 2.2. 15 cells","volume":"43","author":"Kim T. 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