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To better understand the host\u2019s response to infection of<jats:italic>B. pseudomallei<\/jats:italic>, an RNA-Seq technology was used to confirm differentially expressed genes (DEGs) in RAW264.7 cells infected with<jats:italic>B. pseudomallei<\/jats:italic>. In total, 4668 DEGs were identified across three time points (4, 8, and 11 hours after infection). Short Time-Series Expression Miner (STEM) analysis revealed the temporal gene expression profiles and identified seven significant patterns in a total of 26 profiles. Kyoto Encyclopedia of Genes and Genomes (KEGG) was utilized to confirm significantly enriched immune process-associated pathways, and 10 DEGs, including<jats:italic>Ccl9, Ifnb1, Tnf<jats:italic>\u03b1<\/jats:italic>, Ptgs2, Tnfaip3, Zbp1, Ccl5, Ifi202b, Nfkbia,<\/jats:italic>and<jats:italic>Nfkbie<\/jats:italic>, were mapped to eight immune process-associated pathways. Subsequent quantitative real-time PCR assays confirmed that the 10 DEGs were all upregulated during infection. Overall, the results showed that<jats:italic>B. pseudomallei<\/jats:italic>infection can initiate a time-series upregulation of immune process-associated DEGs in RAW264.7 macrophage cells. The discovery of this article helps us better understand the biological function of the immune process-associated genes during<jats:italic>B. pseudomallei<\/jats:italic>infection and may aid in the development of prophylaxis and treatment protocols for melioidosis.<\/jats:p>","DOI":"10.1155\/2018\/1235097","type":"journal-article","created":{"date-parts":[[2018,6,4]],"date-time":"2018-06-04T23:30:39Z","timestamp":1528155039000},"page":"1-9","source":"Crossref","is-referenced-by-count":4,"title":["Upregulation of Immune Process-Associated Genes in RAW264.7 Macrophage Cells in Response to<i>Burkholderia pseudomallei<\/i>Infection"],"prefix":"10.1155","volume":"2018","author":[{"given":"Dongmei","family":"Peng","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Haidian Island, Haikou 570228, 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