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Kartogenin (KGN) was recently identified to improve <jats:italic>in vivo<\/jats:italic> cartilage repair; however, its effect on bone formation is unknown. The aim of this study was to investigate the effect of KGN on antioxidant properties and osteogenic differentiation of bone marrow\u2010derived mesenchymal stem cells (BM\u2010MSCs). Human BM\u2010MSCs were treated with KGN at concentrations ranging from 10<jats:sup>\u22128<\/jats:sup>\u2009M to 10<jats:sup>\u22126<\/jats:sup>\u2009M. Our results indicated that KGN improved cell proliferation and attenuated intracellular reactive oxygen species. The levels of antioxidant enzymes and osteogenic differentiation of BM\u2010MSCs were enhanced by KGN in a dose\u2010dependent manner. Furthermore, KGN\u2010treated BM\u2010MSCs showed upregulation of silent information regulator type 1 (SIRT1) and increased phosphorylation of adenosine 5\u2009<jats:sup>\u2032<\/jats:sup>\u2010monophosphate\u2010activated protein kinase (AMPK), indicating that KGN activated the AMPK\u2010SIRT1 signaling pathway in BM\u2010MSCs. Inhibition of SIRT1 by nicotinamide reversed the antioxidant effect of KGN on BM\u2010MSCs and suppressed osteogenic differentiation. In conclusion, our results demonstrated that KGN improved intracellular antioxidant properties and promoted osteogenic differentiation of BM\u2010MSCs by activating the AMPK\u2010SIRT1 signaling pathway. Thus, KGN may have the potential for not only articular cartilage repair but also the clinical application of MSCs in bone tissue engineering.<\/jats:p>","DOI":"10.1155\/2018\/1368142","type":"journal-article","created":{"date-parts":[[2018,7,15]],"date-time":"2018-07-15T23:30:58Z","timestamp":1531697458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem 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