{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T01:40:59Z","timestamp":1788054059705,"version":"build-2784847793"},"reference-count":13,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["7164255"],"award-info":[{"award-number":["7164255"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Scanning"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p><jats:italic>Purpose<\/jats:italic>. The purpose of this study was to evaluate the role of scanning electron microscopy (SEM) in the direct diagnosis of suspected onychomycosis with negative mycological test results.<jats:italic>Methods<\/jats:italic>. Outpatients diagnosed with suspected onychomycosis with negative mycological test results, including direct microscopic examination with 10% potassium hydroxide (KOH) and fungal culture, on 3 separate occasions were recruited. A small piece of infected nail was obtained for SEM examination.<jats:italic>Results<\/jats:italic>. Among the 48 suspected onychomycosis samples, SEM revealed that 18 (37.5%) were positive for fungal structures, including 10 (20.8%) cases of hyphae and 8 (16.7%) cases of yeast blastospores or budding.<jats:italic>Conclusion<\/jats:italic>. SEM represents an effective method to diagnose suspected onychomycosis when the traditional mycological methods were negative. Therefore, this technique could be used in clinical practice.<\/jats:p>","DOI":"10.1155\/2018\/1581495","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T23:38:38Z","timestamp":1515541118000},"page":"1-4","source":"Crossref","is-referenced-by-count":6,"title":["The Role of Scanning Electron Microscopy in the Direct Diagnosis of Onychomycosis"],"prefix":"10.1155","volume":"2018","author":[{"given":"Xueping","family":"Yue","sequence":"first","affiliation":[{"name":"Department of Dermatology, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4392-3142","authenticated-orcid":true,"given":"Aiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology & Venereology, Peking University First Hospital and Research Center for Medical Mycology, Peking University, Beijing 100034, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7677-2435","authenticated-orcid":true,"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Beijing Tian Tan Hospital, Capital Medical University, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12251"},{"issue":"10","key":"2","first-page":"641","volume":"16","year":"2006","journal-title":"Journal of the College of Physicians and Surgeons Pakistan"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12471"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s00105-013-2562-9"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.1981.tb01020.x"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2010.03.011"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2009.04251.x"},{"issue":"4","key":"8","first-page":"220","volume":"30","year":"2001","journal-title":"Clinics in Dermatology"},{"issue":"5","key":"9","first-page":"315","volume":"31","year":"1998","journal-title":"Chinese Journal of Dermatology"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822013005000056"},{"issue":"2","key":"11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.33073\/pjm-2012-016","volume":"61","year":"2012","journal-title":"Polish Journal of Microbiology"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1002\/sca.21252"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1240-1"}],"container-title":["Scanning"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/scanning\/2018\/1581495.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/scanning\/2018\/1581495.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/scanning\/2018\/1581495.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,25]],"date-time":"2020-10-25T11:30:38Z","timestamp":1603625438000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/scanning\/2018\/1581495\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":13,"alternative-id":["1581495","1581495"],"URL":"https:\/\/doi.org\/10.1155\/2018\/1581495","relation":{},"ISSN":["0161-0457","1932-8745"],"issn-type":[{"value":"0161-0457","type":"print"},{"value":"1932-8745","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}