{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T16:21:29Z","timestamp":1772900489116,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T00:00:00Z","timestamp":1543363200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["RC21-2015"],"award-info":[{"award-number":["RC21-2015"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2018,11,28]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Alterations of the small-intestinal permeability (s-IP) might play an essential role in a subgroup of diarrhoea-predominant IBS (D-IBS) patients. <jats:italic>Goals<\/jats:italic>. (<jats:italic>a<\/jats:italic>) To analyse in D-IBS patients the symptom profile in relation to the altered (+) or not (\u2212) s-IP using the Gastrointestinal Symptom Rating Scale (GSRS). (<jats:italic>b<\/jats:italic>) To assess the circulating levels of the adipokines IL-6, IL-8, TNF-<jats:italic>\u03b1<\/jats:italic>, leptin, and adiponectin, along with LPS, TLR-4, neurotensin, and brain-derived neurotrophic factor (BDNF). The frequency distribution of SNPs at the loci for the investigated molecules and leptin receptor was evaluated. <jats:italic>Study<\/jats:italic>. The study included 34 D-IBS patients and 17 healthy controls (HC). s-IP permeability was assayed by high-performance liquid chromatography determination in the urine of the lactulose to mannitol ratio. Concentrations of IL-6, IL-8, TNF-<jats:italic>\u03b1<\/jats:italic>, LPS, TLR-4, leptin, adiponectin, neurotensin, and BDNF were assayed by ELISA. Screening of genetic variants was done employing the restriction fragment length polymorphism-polymerase chain reaction method. <jats:italic>Results<\/jats:italic>. D-IBS(\u2212) patients had a significantly higher GSRS cluster pain and diarrhoea profile than D-IBS(+) ones. Significant correlations were found between the symptoms clusters and immune activation and inflammation markers. The levels of adipo(cyto)kines in D-IBS(+) patients were higher than those of controls, and IL-6 levels correlated with those of LPS. Leptin and BDNF were significantly higher, and neurotensin levels were significantly lower in D-IBS(+) than in controls. No differences were found in the frequency distribution of genotypes among the study groups. <jats:italic>Conclusions<\/jats:italic>. Results from this study could be of some help in the characterization of the D-IBS and highlight the contribution of an altered intestinal barrier in the pathogenesis of this syndrome. Besides, a role could be ascribed to molecules secreted by the visceral adipose tissue that can impact on barrier functions.<\/jats:p>","DOI":"10.1155\/2018\/1827937","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T18:30:24Z","timestamp":1543429824000},"page":"1-10","source":"Crossref","is-referenced-by-count":27,"title":["Adipose Tissue-Derived Biomarkers of Intestinal Barrier Functions for the Characterization of Diarrhoea-Predominant IBS"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0538-6072","authenticated-orcid":true,"given":"Francesco","family":"Russo","sequence":"first","affiliation":[{"name":"Laboratory of Nutritional Pathophysiology, National Institute of Gastroenterology \u201cS. de Bellis\u201d, Research Hospital, Castellana Grotte Bari, 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