{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:29:51Z","timestamp":1777724991342,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470202"],"award-info":[{"award-number":["81470202"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570288"],"award-info":[{"award-number":["81570288"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470202"],"award-info":[{"award-number":["81470202"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570288"],"award-info":[{"award-number":["81570288"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>Primary ciliary dyskinesia (PCD) is a clinical rare peculiar disorder, mainly featured by respiratory infection, tympanitis, nasosinusitis, and male infertility. Previous study demonstrated it is an autosomal recessive disease and by 2017 almost 40 pathologic genes have been identified. Among them are the leucine-rich repeat- (LRR-) containing 6 (LRRC6) codes for a 463-amino-acid cytoplasmic protein, expressed distinctively in motile cilia cells, including the testis cells and the respiratory epithelial cells. In this study, we applied whole-exome sequencing combined with PCD-known genes filtering to explore the genetic lesion of a PCD patient. A novel compound heterozygous mutation in<jats:italic> LRRC6<\/jats:italic> (c.183T&gt;G\/p.N61K; c.179-1G&gt;A) was identified and coseparated in this family. The missense mutation (c.183T&gt;G\/p.N61K) may lead to a substitution of asparagine by lysine at position 61 in exon 3 of<jats:italic> LRRC6<\/jats:italic>. The splice site mutation (c.179-1G&gt;A) may cause a premature stop codon in exon 4 and decrease the mRNA levels of<jats:italic> LRRC6<\/jats:italic>. Both mutations were not present in our 200 local controls, dbSNP, and 1000 genomes. Three bioinformatics programs also predicted that both mutations are deleterious. Our study not only further supported the importance of LRRC6 in PCD, but also expanded the spectrum of<jats:italic> LRRC6<\/jats:italic> mutations and will contribute to the genetic diagnosis and counseling of PCD patients.<\/jats:p>","DOI":"10.1155\/2018\/1854269","type":"journal-article","created":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T18:30:42Z","timestamp":1515436242000},"page":"1-5","source":"Crossref","is-referenced-by-count":12,"title":["Whole-Exome Sequencing Identified a Novel Compound Heterozygous Mutation of<i> LRRC6<\/i> in a Chinese Primary Ciliary Dyskinesia Patient"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9719-9930","authenticated-orcid":true,"given":"Lv","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, Diagnosis and Treatment Center of Respiratory Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1515-4339","authenticated-orcid":true,"given":"Hong","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Diagnosis and Treatment Center of Respiratory Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.02143-2016"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201607-1351OC"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2015-207891"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201409-1672OC"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcx083"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2017.00135"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2016.11.019"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14279"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2016.06.022"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2016.06.014"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2012.10.003"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23261"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1300968110"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059436"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12014"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1159\/000455181"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.25011\/cim.v33i1.11833"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.3109\/08860221003646337"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08510-z"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.3109\/02713680903503512"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s00586-015-3999-6"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1534\/g3.116.033696"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.5.1278"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1111\/gtc.12380"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00052014"},{"issue":"2","key":"26","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.ajhg.2013.07.009","volume":"93","year":"2013","journal-title":"American Journal of Human Genetics"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2018\/1854269.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2018\/1854269.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2018\/1854269.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,1,8]],"date-time":"2018-01-08T18:30:47Z","timestamp":1515436247000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2018\/1854269\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":26,"alternative-id":["1854269","1854269"],"URL":"https:\/\/doi.org\/10.1155\/2018\/1854269","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}