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As the initial barrier to environmental pollutants and allergic stimuli, airway epithelial cell is vulnerable to oxidative stress. In recent years, the antioxidant effect of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) has attracted much attention. Therefore, in this study, we explored the impact of H<jats:sub>2<\/jats:sub>S on CoCl<jats:sub>2<\/jats:sub>-induced cell injury in 16HBE14o- cells. The effect of CoCl<jats:sub>2<\/jats:sub>on the cell viability was detected by Cell Counting Kit (CCK-8) and the level of ROS in 16HBE14o- cells in response to varying doses (100\u20131000<jats:italic><jats:italic>\u03bc<\/jats:italic><\/jats:italic>mol\/L) of CoCl<jats:sub>2<\/jats:sub>(a common chemical mimic of hypoxia) was measured by using fluorescent probe DCFH-DA. It was shown that, in 16HBE14o- cells, CoCl<jats:sub>2<\/jats:sub>acutely increased the ROS content in a dose-dependent manner, and the increased ROS was inhibited by the NaHS (as a donor of H<jats:sub>2<\/jats:sub>S). Moreover, the calcium ion fluorescence probe Fura-2\/AM and fluorescence dye Rh123 were used to investigate the intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup><mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mo stretchy=\"false\">]<\/mml:mo><\/mml:mrow><mml:mrow><mml:mi mathvariant=\"normal\">i<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) and mitochondria membrane potential (MMP) in 16HBE14o- cells, respectively. In addition, we examined apoptosis of 16HBE14o- cells with Hoechst 33342. The results showed that the CoCl<jats:sub>2<\/jats:sub>effectively elevated the Ca<jats:sup>2+<\/jats:sup>influx, declined the MMP, and aggravated apoptosis, which were abrogated by NaHS. These results demonstrate that H<jats:sub>2<\/jats:sub>S could attenuate CoCl<jats:sub>2<\/jats:sub>-induced hypoxia injury via reducing ROS to perform an agonistic role for the Ca<jats:sup>2+<\/jats:sup>influx and MMP dissipation.<\/jats:p>","DOI":"10.1155\/2018\/2070971","type":"journal-article","created":{"date-parts":[[2018,9,30]],"date-time":"2018-09-30T23:30:57Z","timestamp":1538350257000},"page":"1-9","source":"Crossref","is-referenced-by-count":20,"title":["Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury via Attenuation of ROS-Mediated Ca<sup>2+<\/sup>Overload and Mitochondrial Dysfunction in Human Bronchial Epithelial Cells"],"prefix":"10.1155","volume":"2018","author":[{"given":"Cai-Xia","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Physiology, Xiangya School of Medicine, Central South University, Changsha, 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