{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:17:11Z","timestamp":1783444631376,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001377","name":"Walther Cancer Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001377","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Oncology"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p>We performed RNA-seq on an Illumina platform for 7 patients with endometrioid endometrial carcinoma for which both tumor tissue and adjacent noncancer tissue were available. A total of 66 genes were differentially expressed with significance level at adjusted <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>p<\/mml:mi><\/mml:mrow><\/mml:math> value &lt; 0.01. Using the gene functional classification tool in the NIH DAVID bioinformatics resource, 5 genes were found to be the only enriched group out of that list of genes. The gene<jats:italic> IGSF9 <\/jats:italic>was chosen for further characterization with immunohistochemical staining of a larger cohort of human endometrioid carcinoma tissues. The expression level of IGSF9 in cancer cells was significantly higher than that in control glandular cells in paired tissue samples from the same patients (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.008<\/mml:mn><\/mml:math>) or in overall comparison between cancer and the control (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.003<\/mml:mn><\/mml:math>). IGSF9 expression is higher in patients with myometrium invasion relative to those without invasion (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn fontstyle=\"italic\">0.015<\/mml:mn><\/mml:math>). Reanalysis of RNA-seq dataset from The Cancer Genome Atlas shows higher expression of IGSF9 in endometrial cancer versus normal control and expression was associated with poor prognosis. These results suggest IGSF9 as a new biomarker in endometrial cancer and warrant further studies on its function, mechanism of action, and potential clinical utility.<\/jats:p>","DOI":"10.1155\/2018\/2439527","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T18:36:16Z","timestamp":1518633376000},"page":"1-13","source":"Crossref","is-referenced-by-count":15,"title":["RNA-seq Reveals the Overexpression of IGSF9 in Endometrial Cancer"],"prefix":"10.1155","volume":"2018","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7503-2953","authenticated-orcid":true,"given":"Zonggao","family":"Shi","sequence":"first","affiliation":[{"name":"Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USA"},{"name":"Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Li","sequence":"additional","affiliation":[{"name":"Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USA"},{"name":"Department of Gynecology and Obstetrics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Tarwater","sequence":"additional","affiliation":[{"name":"Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, South Bend, IN 46617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Kaliney","sequence":"additional","affiliation":[{"name":"Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USA"},{"name":"Department of Pathology, The Medical Foundation, South Bend, IN 46601, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darshan S.","family":"Chandrashekar","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama School of Medicine, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Sharon","family":"Stack","sequence":"additional","affiliation":[{"name":"Harper Cancer Research Institute, University of Notre Dame, South Bend, IN 46617, USA"},{"name":"Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, IN 46617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.079558.108"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.5114\/wo.2014.47136"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2017311"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2455"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21262"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/nature12113"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1159\/000445073"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110163"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.099"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1039\/C5MB00663E"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2017.05.002"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-011-0885-3"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.4103\/2395-3977.163802"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-9-r183"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2002.6757"},{"key":"19","year":"2012"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0881-8"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2956"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2016.02.003"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbw069"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00916-z"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.14717"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1586\/era.12.158"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1097\/IGC.0b013e318218f28b"},{"issue":"5","key":"28","first-page":"514","volume":"30","year":"2009","journal-title":"Eur J Gynaecol Oncol"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-2-r14"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405370101"}],"container-title":["Journal of Oncology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jo\/2018\/2439527.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jo\/2018\/2439527.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jo\/2018\/2439527.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T18:36:19Z","timestamp":1518633379000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jo\/2018\/2439527\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":29,"alternative-id":["2439527","2439527"],"URL":"https:\/\/doi.org\/10.1155\/2018\/2439527","relation":{},"ISSN":["1687-8450","1687-8469"],"issn-type":[{"value":"1687-8450","type":"print"},{"value":"1687-8469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}