{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T23:03:00Z","timestamp":1772060580970,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010029","name":"Lancaster University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010029","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006507","name":"FIOCRUZ","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2018]]},"abstract":"<jats:p><jats:italic>Lutzomyia longipalpis<\/jats:italic> is the main vector of visceral leishmaniasis (VL) in America. Physiological and molecular mechanisms of<jats:italic> Leishmania<\/jats:italic> infection in sand flies have been studied during the first gonotrophic cycle. There are few studies about these interactions during the second gonotrophic cycle mainly because of the difficulties maintaining sand flies through sequential feeds. Here we standardized conditions to perform the second blood feed efficiently, and our results show that oviposition is an essential factor for the success of multiple feeds. We evaluated the impact of the second blood meal on longevity, protein digestion, trypsin activity, and<jats:italic> Leishmania mexicana<\/jats:italic> development within<jats:italic> L. longipalpis<\/jats:italic> gut. Mortality of blood-fed females increases after second blood meal as compared to sugar-fed females. Trypsin activity was lower during the second gonotrophic cycle. However, no difference in protein intake was observed between blood meals. There was no difference in the population size of<jats:italic> Leishmania<\/jats:italic> in the gut after both blood meals. In this work, we presented an optimized protocol for obtaining sufficient numbers of sand fly females fed on a second blood meal, and we described some physiological and parasitological aspects of the second gonotrophic cycle which might influence the vectorial competence of sand flies.<\/jats:p>","DOI":"10.1155\/2018\/2472508","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T19:30:41Z","timestamp":1522006241000},"page":"1-10","source":"Crossref","is-referenced-by-count":30,"title":["Second Blood Meal by Female<i> Lutzomyia longipalpis<\/i>: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and<i> Leishmania<\/i> Infection"],"prefix":"10.1155","volume":"2018","author":[{"given":"C. S.","family":"Moraes","sequence":"first","affiliation":[{"name":"Laboratory of Insect Biochemistry and Physiology, Oswaldo Cruz Institute, FIOCRUZ, 4365 Brasil Av., Leonidas Deane Building, Room 207, 21040-360 Manguinhos, RJ, Brazil"},{"name":"Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Furness Building, Bailrigg, Lancaster LA1 4YG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Aguiar-Martins","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Furness Building, Bailrigg, Lancaster LA1 4YG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. G.","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Insect Biochemistry and Physiology, Oswaldo Cruz Institute, FIOCRUZ, 4365 Brasil Av., Leonidas Deane Building, Room 207, 21040-360 Manguinhos, RJ, Brazil"},{"name":"Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Furness Building, Bailrigg, Lancaster LA1 4YG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6861-5421","authenticated-orcid":true,"given":"P. A.","family":"Bates","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Furness Building, Bailrigg, Lancaster LA1 4YG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Dillon","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Furness Building, Bailrigg, Lancaster LA1 4YG, UK"},{"name":"National Institute of Science and Technology for Molecular Entomology, 373 Carlos Chagas Filho Av., Center for Health Science, Building D, Basement, Room 5, Cidade Universit\u00e1ria, 21941-590 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9558-1116","authenticated-orcid":true,"given":"F. A.","family":"Genta","sequence":"additional","affiliation":[{"name":"Laboratory of Insect Biochemistry and Physiology, Oswaldo Cruz Institute, FIOCRUZ, 4365 Brasil Av., Leonidas Deane Building, Room 207, 21040-360 Manguinhos, RJ, Brazil"},{"name":"National Institute of Science and Technology for Molecular Entomology, 373 Carlos Chagas Filho Av., Center for Health Science, Building D, Basement, Room 5, Cidade Universit\u00e1ria, 21941-590 Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2012.03.005"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1590\/s0074-02762005000800001"},{"key":"4","year":"1994"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585(2005)042[0928:LLPMFS]2.0.CO;2"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/45.1.160"},{"key":"7"},{"key":"8","year":"1984"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000049015"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-329"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.1987.tb00349.x"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931590"},{"key":"13","first-page":"275","volume-title":"Intera\u00e7\u00e3o Vetor-Hospedeiro","year":"2003"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.06.012"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-2-62"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010697"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111241"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00033-6"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01132.x"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013000358"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1983.tb02923.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1006\/expr.2000.4569"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4894(03)00031-6"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2005.10.005"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.2174\/1566524043360285"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(86)90045-7"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1007\/BF00932379"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762001000300001"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001288"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90442-7"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/52.3-4.591"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-009-0802-1"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/S0171-9335(99)80036-3"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1038\/nature02675"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.01.009"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/arch.20198"},{"key":"38","first-page":"171","volume-title":"Biochemistr of digestion in Comprehensive Molecular Insect Science","volume":"4","year":"2005"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(91)90050-O"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2010.01439.x"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002001439"},{"key":"42","volume":"8","year":"2013","journal-title":"PLoSOne"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2018\/2472508.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2018\/2472508.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2018\/2472508.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T12:47:31Z","timestamp":1526647651000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/bmri\/2018\/2472508\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018]]},"references-count":41,"alternative-id":["2472508","2472508"],"URL":"https:\/\/doi.org\/10.1155\/2018\/2472508","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018]]}}}